Antibodies, Proteins, Kits and Reagents for Life Science

328 339 ₸ Product size

100 µl 328 339 ₸

Order now and get it on Tuesday March 02, 2021

Anti-ROR gamma antibody [EPR20006] (ab207082)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20006] to ROR gamma
Suitable for: Flow Cyt, IHC-P, WB, IP
Reacts with: Mouse

Product name

Anti-ROR gamma antibody [EPR20006]
See all ROR gamma primary antibodies
Description

Rabbit monoclonal [EPR20006] to ROR gamma
Host species

Rabbit
Specificity

Our data suggests that this antibody is specific to ROR gamma isoform 2 which is known as ROR gammaT.

Tested Applications & Species

Application	Species
Flow Cyt	Mouse
IHC-P	Mouse
IP	Mouse
WB	Mouse

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: His-tagged mouse ROR gamma recombinant protein (aa 1-266); Mouse thymus lysate. IHC-P: Mouse thymus and spleen tissues. Flow Cyt: Mouse thymocytes. IP: Mouse thymus lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20006
Isotype

IgG
Research areas

Flow Cytometry - Anti-ROR gamma antibody [EPR20006] (ab207082)

Flow cytometric analysis of 4% paraformaldehyde-fixed Mouse thymocytes labeling ROR gamma with ab207082 at 1/50 dilution. Goat anti rabbit IgG (Alexa Fluor^® 488) at 1/2000 dilution was used as the secondary antibody.

Note: Cell surface staining with PE conjugated anti-mouse CD8 and Alexa Fluor^® 647 conjugated anti mouse CD4 antibodies, followed by fixation with 4% PFA for 10 min and intracellular staining with ab207082. The image shows thymocytes in the CD4+CD8+ (red) or the CD4+CD8- (black, negative control) gate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROR gamma antibody [EPR20006] (ab207082)

Immunohistochemical analysis of paraffin-embedded mouse thymus tissue labeling ROR gamma with ab207082 at 1/3000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on mouse thymus cortex, located in the left and right of the image, with sporadic nuclear staining on medulla, middle region (PMID:14691482).

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROR gamma antibody [EPR20006] (ab207082)

Immunohistochemical analysis of paraffin-embedded mouse thymus tissue labeling ROR gamma with ab207082 at 1/3000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on the cortical region of mouse thymus shown at a high magnification (400×) (PMID:14691482).

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-ROR gamma antibody [EPR20006] (ab207082)

Lane 1 : Anti-ROR gamma antibody [EPR20006] (ab207082) at 1/10000 dilution
Lanes 2-3 : Anti-ROR gamma antibody [EPR20006] (ab207082) at 1/1000 dilution

Lane 1 : His-tagged mouse ROR gamma recombinant protein (aa 1-266)
Lane 2 : His-tagged mouse ROR alpha recombinant protein (aa 48-215)
Lane 3 : His-tagged mouse ROR beta recombinant protein (aa 9-140)

Lysates/proteins at 0.01 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 58 kDa
Observed band size: 35 kDa
why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: Lane 1: 3 seconds; Lane 2/3: 3 minutes.
Western blot - Anti-ROR gamma antibody [EPR20006] (ab207082)

All lanes : Anti-ROR gamma antibody [EPR20006] (ab207082) at 1/2000 dilution

Lane 1 : Mouse thymus lysate
Lane 2 : Mouse liver lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 58 kDa
Observed band size: 58 kDa

Exposure time: 3 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.

The expression profile is consistent with the literature: PMID 14691482; PMID 9881970.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROR gamma antibody [EPR20006] (ab207082)

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling ROR gamma with ab207082 at 1/3000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Sporadic nuclear staining on mouse spleen (PMID:14691482).

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunoprecipitation - Anti-ROR gamma antibody [EPR20006] (ab207082)

ROR gamma was immunoprecipitated from 0.35 mg of Mouse thymus lysate with ab207082 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab207082 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1: Mouse thymus lysate, 10 μg (Input).

Lane 2: ab207082 IP in Mouse thymus lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab207082 inMouse thymus lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 10 seconds.
Anti-ROR gamma antibody [EPR20006] (ab207082)

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