Antibodies, Proteins, Kits and Reagents for Life Science

288 134 ₸ Product size

100 µl 288 134 ₸ 10 µl 110 563 ₸

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Anti-DPP4 antibody [EPR20819] (ab215711)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20819] to DPP4
Suitable for: ELISA, WB, IHC-P
Reacts with: Human, Recombinant fragment

Product name

Anti-DPP4 antibody [EPR20819]
See all DPP4 primary antibodies
Description

Rabbit monoclonal [EPR20819] to DPP4
Host species

Rabbit

Tested Applications & Species

Application	Species
ELISA	Recombinant fragment
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Caco-2 and LoVo whole cell lysate. Human fetal kidney, fetal lung and fetal liver lysate. IHC-P: Human liver tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20819
Isotype

IgG
Research areas

Western blot - Anti-DPP4 antibody [EPR20819] (ab215711)

All lanes : Anti-DPP4 antibody [EPR20819] (ab215711) at 0.4 µg/ml

Lane 1 : Caco-2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate prepared using RIPA lysis method
Lane 2 : Caco-2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate prepared using 1%SDS hot lysis method

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 88 kDa

Blocking and diluting buffer: 5% NFDM/TBST.

For this product, we recommend 1%SDS hot lysis method.

For lysate preparation protocol, please refer to the protocol book in the protocol section or here (downloadable copy).

Exposure time:
Lane 1: 3 minutes
Lane 2: 7 seconds
Western blot - Anti-DPP4 antibody [EPR20819] (ab215711)

All lanes : Anti-DPP4 antibody [EPR20819] (ab215711) at 1/1000 dilution

Lane 1 : Caco-2 (human colorectal adenocarcinoma epithelial cell), whole cell lysate at 20 µg
Lane 2 : LoVo (human colorectal adenocarcinoma epithelial cell), whole cell lysate at 10 µg
Lane 3 : Human fetal kidney at 10 µg
Lane 4 : Human fetal lung at 10 µg
Lane 5 : Human fetal liver at 10 µg

Secondary
Lane 1 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lane 2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Lanes 3-5 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

Predicted band size: 88 kDa

Blocking: 5% NFDM/TBST.

Exposure times:

Lanes 1,3: 3 minutes

Lane 2: 15 seconds

Lane 4: 1 minute

Lane 5: 30 seconds

The molecular weight observed is consistent with what has been described in the literature (PMID: 25589660).

All the lysates were preparaed using 1%SDS hot lysis method
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPP4 antibody [EPR20819] (ab215711)

Immunohistochemical analysis of paraffin-embedded human liver tissue labeling CD26 with ab215711 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous staining on the bile canaliculi of human liver (PMID: 23361237) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ELISA - Anti-DPP4 antibody [EPR20819] (ab215711)

ELISA analysis of Human DPP4 recombinant protein at 1000 ng/mL with ab215711. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.
Anti-DPP4 antibody [EPR20819] (ab215711)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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