Anti-Surfactant Protein A/PSAP antibody (ab115791)
Key features and details
- Rabbit polyclonal to Surfactant Protein A/PSAP
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-Surfactant Protein A/PSAP antibody
See all Surfactant Protein A/PSAP primary antibodies -
Description
Rabbit polyclonal to Surfactant Protein A/PSAP -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Synthetic peptide corresponding to Mouse Surfactant Protein A/PSAP aa 200-300 (C terminal).
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Positive control
- WB: Mouse and rat lung tissue lysate; IHC-P: Mouse and rat lung tissue.
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General notes
This product was previously labelled as Surfactant Protein A
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Surfactant Protein A/PSAP antibody (ab115791)All lanes : Anti-Surfactant Protein A/PSAP antibody (ab115791) at 0.5 µg/ml
Lanes 1-2 : mouse lung tissue lysates with 5% Non-fat Milk/ TBS for 1.5 hour at RT
Lanes 3-4 : rat lung tissue lysates with 5% Non-fat Milk/ TBS for 1.5 hour at RT
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG-HRP at 1/10000 dilution
Predicted band size: 26 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Surfactant Protein A/PSAP antibody (ab115791)Surfactant Protein A/PSAP was detected in paraffin-embedded section of mouse lung tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml ab115791 overnight at 4°C. Biotinylated goat antirabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Surfactant Protein A/PSAP antibody (ab115791)Surfactant Protein A/PSAP was detected in paraffin-embedded section of rat lung tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml ab115791 overnight at 4°C. Biotinylated goat antirabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.
