Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Surfactant Protein A/PSAP antibody [6F10] (ab51891)

Key features and details

  • Mouse monoclonal [6F10] to Surfactant Protein A/PSAP
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-Surfactant Protein A/PSAP antibody [6F10]
    See all Surfactant Protein A/PSAP primary antibodies

  • Description

    Mouse monoclonal [6F10] to Surfactant Protein A/PSAP

  • Host species

    Mouse

  • Specificity

    ab51891 is specific for human pulmonary Surfactant Protein A/PSAP.

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Full length native protein (purified) corresponding to Human Surfactant Protein A/PSAP.

  • Positive control

    • Human lung tissue

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Surfactant Protein A/PSAP antibody [6F10] (ab51891)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Surfactant Protein A/PSAP antibody [6F10] (ab51891)
    ab51891 staining sections of human lung at 1:3000 dilution.  Immunoreactivity is seen with alveolar type II cells, nonciliated bronchial cells and a subset of alveolar macrophages.
  • Western blot - Anti-Surfactant Protein A/PSAP antibody [6F10] (ab51891)
    Western blot - Anti-Surfactant Protein A/PSAP antibody [6F10] (ab51891)
    All lanes : Anti-Surfactant Protein A/PSAP antibody [6F10] (ab51891) at 1 µg/ml

    Lane 1 : Lung (Human) Tissue Lysate
    Lane 2 : Lung (Human) Whole Cell Lysate - fetal normal tissue (ab30282)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 26 kDa
    Observed band size: 37 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 50 kDa, 63 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 150 seconds


    Surfactant Protein A/PSAP contains a number of potential glycosylation sites and other post translational modifications (SwissProt) which may explain its migration at a higher molecular weight than predicted.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Surfactant Protein A/PSAP antibody [6F10] (ab51891)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Surfactant Protein A/PSAP antibody [6F10] (ab51891)
    ab51891 staining sections of human lung at 1:3000 dilution.  Immunoreactivity is seen with alveolar type II cells, nonciliated bronchial cells and a subset of alveolar macrophages.

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