Antibodies, Proteins, Kits and Reagents for Life Science

304 886 ₸ Product size

100 µl 304 886 ₸

Order now and get it on Wednesday March 10, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR8482] to RRM1
Suitable for: WB, IHC-P
Reacts with: Mouse, Human

Product name

Anti-RRM1 antibody [EPR8482]
See all RRM1 primary antibodies
Description

Rabbit monoclonal [EPR8482] to RRM1
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human RRM1 aa 50-150. The exact sequence is proprietary.
Positive control
- HeLa, HT29, RAW 264.7 and NIH 3T3 cell lysates; Human placenta and kidney tissues.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (K_D)

K_D = 1.24 x 10 ^-11 M
Learn more about K_D
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR8482
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRM1 antibody [EPR8482] (ab133690)

Immunohistochemical analysis of paraffin-embedded Human placenta tissue labelling RRM1 with ab133690 at 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRM1 antibody [EPR8482] (ab133690)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling RRM1 with ab133690 at 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-RRM1 antibody [EPR8482] (ab133690)

All lanes : Anti-RRM1 antibody [EPR8482] (ab133690) at 1/1000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : HT29 cell lysate
Lane 3 : RAW 264.7 cell lysate
Lane 4 : NIH 3T3 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 90 kDa
OI-RD Scanning - Anti-RRM1 antibody [EPR8482] (ab133690)

Equilibrium disassociation constant (K_D)
Learn more about K_D

Click here to learn more about K_D
Anti-RRM1 antibody [EPR8482] (ab133690)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com