Antibodies, Proteins, Kits and Reagents for Life Science

Anti-RRM1 antibody [SP167] - BSA and Azide free (ab240093)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [SP167] to RRM1 - BSA and Azide free
Suitable for: Flow Cyt, IHC-P, ICC/IF
Reacts with: Human

Product name

Anti-RRM1 antibody [SP167] - BSA and Azide free
See all RRM1 primary antibodies
Description

Rabbit monoclonal [SP167] to RRM1 - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: Flow Cyt, IHC-P, ICC/IFmore details
Species reactivity

Reacts with: Human
Predicted to work with: Mouse, Cow
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
General notes
FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Ab240093 is the carrier-free version of ab135383. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab240093 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.20
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A/G purified
Purification notes

Purified from TCS by protein A/G.
Clonality

Monoclonal
Clone number

SP167
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRM1 antibody [SP167] - BSA and Azide free (ab240093)

Immunohistochemical analysis of paraffin embedded human lung adenocarcinoma tissue labeling RRM1 with ab135383 antibody at a dilution of 1/100.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab135383).
Flow Cytometry - Anti-RRM1 antibody [SP167] - BSA and Azide free (ab240093)

Flow cytometric analysis of rabbit anti-RRM1 (SP167) antibody ab135383 (1/400) in HeLa cells (green) compared to negative control of rabbit IgG (blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab135383).
Immunocytochemistry/ Immunofluorescence - Anti-RRM1 antibody [SP167] - BSA and Azide free (ab240093)

ICC/IF image of ab135383 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab135383 at 1/100 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab135383).
Anti-RRM1 antibody [SP167] - BSA and Azide free (ab240093)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com