Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Ferritin Light Chain antibody [EPR5260] - BSA and Azide free (ab239971)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5260] to Ferritin Light Chain - BSA and Azide free
  • Suitable for: Flow Cyt, WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Ferritin Light Chain antibody [EPR5260] - BSA and Azide free
    See all Ferritin Light Chain primary antibodies

  • Description

    Rabbit monoclonal [EPR5260] to Ferritin Light Chain - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, WBmore details
    Unsuitable for: ICC or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa and HAP1 cell lysates. IHC-P: Mouse brain tissue. Flow Cyt: HeLa cells.

  • General notes

    Ab239971 is the carrier-free version of ab109373. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab239971 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Ferritin Light Chain antibody [EPR5260] - BSA and Azide free (ab239971)
    Western blot - Anti-Ferritin Light Chain antibody [EPR5260] - BSA and Azide free (ab239971)
    All lanes : Anti-Ferritin Light Chain antibody [EPR5260] (ab109373) at 1/2000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : Ferritin knockout HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 20 kDa



    This data was developed using the same antibody clone in a different buffer formulation (ab109373).

    Lanes 1-2: Merged signal (red and green). Green - ab109373 observed at 20 kDa. Red - loading control ab8245 observed at 37 kDa.

     ab109373 Anti-FTL was shown to specifically react with Ferritin in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265533 (knockout cell lysate ab256926) was used. Wild-type and FTL knockout samples were subjected to SDS-PAGE. ab109373 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 2000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

     

  • Western blot - Anti-Ferritin Light Chain antibody [EPR5260] - BSA and Azide free (ab239971)
    Western blot - Anti-Ferritin Light Chain antibody [EPR5260] - BSA and Azide free (ab239971)
    All lanes : Anti-Ferritin Light Chain antibody [EPR5260] (ab109373) at 1/10000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : FTL (Ferritin Light Chain) knockout HAP1 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 20 kDa



    Lanes 1 - 2: Merged signal (red and green). Green - ab109373 observed at 20 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab109373 was shown to recognize Ferritin Light Chain in wild-type HAP1 cells as signal was lost at the expected MW in FTL (Ferritin Light Chain) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and FTL (Ferritin Light Chain) knockout samples were subjected to SDS-PAGE. Ab109373 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109373).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin Light Chain antibody [EPR5260] - BSA and Azide free (ab239971)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin Light Chain antibody [EPR5260] - BSA and Azide free (ab239971)

    Immunohistochemical analysis of wild type and ftl knock out mouse brain tissue labeling Ferritin Light Chain. Positive staining of Ferratin Light Chain is seen in wild type mouse brain (A) and no staining is seen in the ftl knock out brain tissue (B).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109373).

  • Flow Cytometry - Anti-Ferritin Light Chain antibody [EPR5260] - BSA and Azide free (ab239971)
    Flow Cytometry - Anti-Ferritin Light Chain antibody [EPR5260] - BSA and Azide free (ab239971)
    Flow cytometric analysis of permeabilized HeLa cells using ab109373 at a dilution of 1/100 (red) or a rabbit IgG (negative) (green).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109373).

  • Anti-Ferritin Light Chain antibody [EPR5260] - BSA and Azide free (ab239971)
    Anti-Ferritin Light Chain antibody [EPR5260] - BSA and Azide free (ab239971)

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