Anti-Ferritin Light Chain antibody [8E1E7] (ab201975)
Key features and details
- Mouse monoclonal [8E1E7] to Ferritin Light Chain
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Ferritin Light Chain antibody [8E1E7]
See all Ferritin Light Chain primary antibodies -
Description
Mouse monoclonal [8E1E7] to Ferritin Light Chain -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Human -
Immunogen
Full length protein corresponding to Human Ferritin Light Chain.
Database link: P02792 -
Positive control
- Recombinant Ferritin Light Chain protein, Ferritin Light Chain transfected HEK293 cell lysate, K562 cell lysate, A431 cells. Human liver cancer tissue and esophageal tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
8E1E7 -
Isotype
IgG1 -
Research areas
Images
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Anti-Ferritin Light Chain antibody [8E1E7] (ab201975) at 1/500 dilution + recombinant human Ferritin Light Chain protein
Predicted band size: 20 kDa
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All lanes : Anti-Ferritin Light Chain antibody [8E1E7] (ab201975) at 1/500 dilution
Lane 1 : HEK293 cell lysate
Lane 2 : Ferritin Light Chain transfected HEK293 cell lysate
Predicted band size: 20 kDa
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Anti-Ferritin Light Chain antibody [8E1E7] (ab201975) at 1/500 dilution + K562 cell lysate
Predicted band size: 20 kDa
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Immunofluorescence analysis of A431 cells labeling Ferritin Light Chain using ab201975 at 1/200 dilution. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin Light Chain antibody [8E1E7] (ab201975)
Immunohistochemical analysis of paraffin-embedded Human liver cancer tissues labeling Ferritin Light Chain using ab201975 at 1/200 dilution with DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin Light Chain antibody [8E1E7] (ab201975)
Immunohistochemical analysis of paraffin-embedded Human esophageal tissue labeling Ferritin Light Chain with ab201975 at 1/200 dilution with DAB staining