Anti-Ferritin Light Chain antibody [CPT-R10-58-11] (ab243096)
![Anti-Ferritin Light Chain antibody [CPT-R10-58-11] (ab243096)](https://www.abcam.com/ps/products/243/ab243096/Images/ab243096-336112-anti-ferritin-light-chain-antibody-cpt-r10-58-11-western-blot.jpg "Anti-Ferritin Light Chain antibody [CPT-R10-58-11] (ab243096)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [CPT-R10-58-11] to Ferritin Light Chain
- Suitable for: WB, Flow Cyt, ELISA
- Reacts with: Human
Overview
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Product name
Anti-Ferritin Light Chain antibody [CPT-R10-58-11]
See all Ferritin Light Chain primary antibodies -
Description
Rabbit monoclonal [CPT-R10-58-11] to Ferritin Light Chain -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: NCI-H460, HEK-293T, MCF7 and HepG2 whole cell lysates; Human brain tissue lysate. Flow Cyt: HepG2 cells. ELISA: CPTAC-14d peptide.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
CPT-R10-58-11 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Ferritin Light Chain antibody [CPT-R10-58-11] (ab243096)All lanes : Anti-Ferritin Light Chain antibody [CPT-R10-58-11] (ab243096) at 1/1000 dilution
Lane 1 : NCI-H460 (human lung cancer cell line) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 5 : Human brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 19 kDa
Observed band size: 19 kDaExposure time : Lane 1-4: 26 seconds, Lane 5: 5.5 seconds.
Blocking/Dilution buffer: 5% NFDM/TBST.
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![Flow Cytometry - Anti-Ferritin Light Chain antibody [CPT-R10-58-11] (ab243096)](https://www.abcam.com/ps/products/243/ab243096/Images/ab243096-336113-anti-ferritin-light-chain-antibody-cpt-r10-58-11-flow-cytometry.jpg)
Flow Cytometry - Anti-Ferritin Light Chain antibody [CPT-R10-58-11] (ab243096)Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cell line labeling Ferritin Light Chain with ab243096 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.
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![ELISA - Anti-Ferritin Light Chain antibody [CPT-R10-58-11] (ab243096)](https://www.abcam.com/ps/products/243/ab243096/Images/ab243096-336114-anti-ferritin-light-chain-antibody-cpt-r10-58-11-elisa.jpg)
ELISA - Anti-Ferritin Light Chain antibody [CPT-R10-58-11] (ab243096)50ng CPTAC-14d (1000ng/mL, 50μL per well) was coated onto 96-wells. Serial dilutions (0, 0.2, 0.9, 3.9, 15, 62, 250, 1000ng/mL) of ab243096 (50μL) were incubated with CPTAC-14d in each well for 60 minutes by shaking. This was followed by adding goat anti-rabbit IgG, (H+L), phosphatase-conjugated secondary antibody (50μL) into each well and incubating for another 40 minutes. After washing, 50μL of PNPP was added and incubated for 15 minutes without shaking for color development. OD was read at 405nm within 5 minutes.
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![Anti-Ferritin Light Chain antibody [CPT-R10-58-11] (ab243096)](https://www.abcam.com/ps/products/243/ab243096/Images/ab243096-4-benefits-of-recombinant-antibodies.png)
Anti-Ferritin Light Chain antibody [CPT-R10-58-11] (ab243096)
