Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Ferritin Light Chain antibody [CPT-R10-58-11] - BSA and Azide free (ab245128)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [CPT-R10-58-11] to Ferritin Light Chain - BSA and Azide free
  • Suitable for: ELISA, WB, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-Ferritin Light Chain antibody [CPT-R10-58-11] - BSA and Azide free
    See all Ferritin Light Chain primary antibodies

  • Description

    Rabbit monoclonal [CPT-R10-58-11] to Ferritin Light Chain - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ELISA, WB, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-Fr or IHC-P

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Flow Cyt: HepG2 cells. ELISA: CPTAC-14d peptide.

  • General notes

    ab245128 is the carrier-free version of ab241329. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab245128 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Flow Cytometry - Anti-Ferritin Light Chain antibody [CPT-R10-58-11] - BSA and Azide free (ab245128)
    Flow Cytometry - Anti-Ferritin Light Chain antibody [CPT-R10-58-11] - BSA and Azide free (ab245128)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cell line labeling Ferritin Light Chain with ab243096 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243096).
  • ELISA - Anti-Ferritin Light Chain antibody [CPT-R10-58-11] - BSA and Azide free (ab245128)
    ELISA - Anti-Ferritin Light Chain antibody [CPT-R10-58-11] - BSA and Azide free (ab245128)

    50ng CPTAC-14d (1000ng/mL, 50μL per well) was coated onto 96-wells. Serial dilutions (0, 0.2, 0.9, 3.9, 15, 62, 250, 1000ng/mL) of ab243096 (50μL) were incubated with CPTAC-14d in each well for 60 minutes by shaking. This was followed by adding goat anti-rabbit IgG, (H+L), phosphatase-conjugated secondary antibody (50μL) into each well and incubating for another 40 minutes. After washing, 50μL of PNPP was added and incubated for 15 minutes without shaking for color development. OD was read at 405nm within 5 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243096).
  • Anti-Ferritin Light Chain antibody [CPT-R10-58-11] - BSA and Azide free (ab245128)
    Anti-Ferritin Light Chain antibody [CPT-R10-58-11] - BSA and Azide free (ab245128)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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