Anti-Cleaved NEAS (Asp1185) antibody [EPR3830-17] - BSA and Azide free (ab247550)
![Anti-Cleaved NEAS (Asp1185) antibody [EPR3830-17] - BSA and Azide free (ab247550)](https://www.abcam.com/ps/products/247/ab247550/Images/ab247550-397989-anti-cleaved-neas-asp1185-antibody-epr3830-17-bsa-and-azide-free-western-blot.jpg "Anti-Cleaved NEAS (Asp1185) antibody [EPR3830-17] - BSA and Azide free (ab247550)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3830-17] to Cleaved NEAS (Asp1185) - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-Cleaved NEAS (Asp1185) antibody [EPR3830-17] - BSA and Azide free
See all Cleaved NEAS (Asp1185) primary antibodies -
Description
Rabbit monoclonal [EPR3830-17] to Cleaved NEAS (Asp1185) - BSA and Azide free -
Host species
Rabbit -
Specificity
Only detects the large fragment of Alpha Fodrin resulting from cleavage at aspartic acid 1185.
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Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab247550 is the carrier-free version of ab92373 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab247550 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information. This product was previously labelled as Alpha Fodrin, Cleaved alpha-fodrin (Asp1185)
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR3830-17 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Cleaved NEAS (Asp1185) antibody [EPR3830-17] - BSA and Azide free (ab247550)All lanes : Anti-Cleaved NEAS (Asp1185) antibody [EPR3830-17] (ab92373)
Lane 1 : HeLa + Camptothecin cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat + Staurosporine cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 285 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?This data was developed using ab92373, the same antibody clone in a different buffer formulation.
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![Anti-Cleaved NEAS (Asp1185) antibody [EPR3830-17] - BSA and Azide free (ab247550)](https://www.abcam.com/ps/products/247/ab247550/Images/ab247550-2-benefits-of-recombinant-antibodies.png)
Anti-Cleaved NEAS (Asp1185) antibody [EPR3830-17] - BSA and Azide free (ab247550)
