Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-n-Myc/MYCN antibody [EPR18982-8R-3] - BSA and Azide free (ab236459)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18982-8R-3] to n-Myc/MYCN - BSA and Azide free
  • Suitable for: WB, IP, ChIP
  • Reacts with: Human

Overview

  • Product name

    Anti-n-Myc/MYCN antibody [EPR18982-8R-3] - BSA and Azide free
    See all n-Myc/MYCN primary antibodies

  • Description

    Rabbit monoclonal [EPR18982-8R-3] to n-Myc/MYCN - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, ChIPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: IMR-32 whole cell lysate.

  • General notes

    ab236459 is the carrier-free version of ab227822 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab236459 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as n-Myc

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-n-Myc/MYCN antibody [EPR18982-8R-3] - BSA and Azide free (ab236459)
    Western blot - Anti-n-Myc/MYCN antibody [EPR18982-8R-3] - BSA and Azide free (ab236459)
    All lanes : Anti-n-Myc/MYCN antibody [EPR18982-8R-3] - ChIP Grade (ab227822) at 1/1000 dilution

    Lane 1 : IMR-32 (human neuroblastoma neuroblast cell line) whole cell lysate
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 50 kDa
    Observed band size: 62 kDa
    why is the actual band size different from the predicted?


    Exposure time: 103 seconds


    Blocking and dilution buffer: 5% NFDM/TBST 

    The expression profile observed is consistent with what has been described in the literature (PMID: 11034201; PMID: 27197171; PMID: 23792191).

    Negative control: HeLa (PMID: 27197171).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227822).

     

  • ChIP - Anti-n-Myc/MYCN antibody [EPR18982-8R-3] - BSA and Azide free (ab236459)
    ChIP - Anti-n-Myc/MYCN antibody [EPR18982-8R-3] - BSA and Azide free (ab236459)

    Chromatin was prepared from IMR-32 cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The ChIP was performed with 25 µg of chromatin, 5 µg of ab227822 (red), and 20 µl of Protein A/G sepharose beads. 5 μg of rabbit normal IgG was added to the beads control (gray). The immunoprecipitated DNA was quantified by real time PCR (SYBR green approach). Primers and probes are located in the first kb of the transcribed region.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227822).

  • Immunoprecipitation - Anti-n-Myc/MYCN antibody [EPR18982-8R-3] - BSA and Azide free (ab236459)
    Immunoprecipitation - Anti-n-Myc/MYCN antibody [EPR18982-8R-3] - BSA and Azide free (ab236459)

    n-Myc/MYCN was immunoprecipitated from 0.35 mg of IMR-32 (human neuroblastoma neuroblast cell line) whole cell lysate with ab227822 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab227822 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1: IMR-32 whole cell lysate 10 μg (Input).

    Lane 2: ab227822 IP in IMR-32 whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab227822 in IMR-32 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 3 seconds.

    The expression profile observed is consistent with what has been described in the literature (PMID: 17938259; PMID: 2657399).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227822).

  • Anti-n-Myc/MYCN antibody [EPR18982-8R-3] - BSA and Azide free (ab236459)
    Anti-n-Myc/MYCN antibody [EPR18982-8R-3] - BSA and Azide free (ab236459)

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