Anti-n-Myc/MYCN antibody [NCM II 100] (ab16898)
![Anti-n-Myc/MYCN antibody [NCM II 100] (ab16898)](https://www.abcam.com/ps/products/16/ab16898/Images/ab16898-25782-anti-n-myc-antibody-ncm-ii-100-chip-grade-immunoprecipitation.jpg "Anti-n-Myc/MYCN antibody [NCM II 100] (ab16898)")
Key features and details
- Mouse monoclonal [NCM II 100] to n-Myc/MYCN
- Suitable for: IP, Flow Cyt
- Reacts with: Mouse, Human
- Isotype: IgG1
Overview
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Product name
Anti-n-Myc/MYCN antibody [NCM II 100]
See all n-Myc/MYCN primary antibodies -
Description
Mouse monoclonal [NCM II 100] to n-Myc/MYCN -
Host species
Mouse -
Specificity
This antibody reacts with N-myc/MYCN encoded proteins and their cleavage products.
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Tested applications
Suitable for: IP, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Fusion protein corresponding to Human n-Myc/MYCN. Recombinant fusion protein. Original clone reference: PubMed ID - 2426708.
Database link: P04198 -
Positive control
- IMR5 Cells
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General notes
It is important to compensate for the short half life of this protein when preparing tissue sections or extracts. We recommended that all preparations be kept cold and that a cocktail of protease inhibitors be used.
This product was previously labelled as n-Myc
This product was changed from ascites to tissue culture supernatant on 17 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituents: 0.82% Sodium phosphate, 50% Glycerol -
Concentration information loading... -
Purity
Tissue culture supernatant -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
NCM II 100 -
Myeloma
Sp2/0 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Immunoprecipitation - Anti-n-Myc/MYCN antibody [NCM II 100] (ab16898)
Immunoprecipitation of N-Myc/MYCN from S35 methionine-labeled IMR-5 cells using ab16898 (lane 1) and Normal Mouse IgG (lane 2).
ab16898 used at 1 mg/sample.This image was generated using the ascites version of the product.
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![Flow Cytometry - Anti-n-Myc/MYCN antibody [NCM II 100] (ab16898)](https://www.abcam.com/ps/products/16/ab16898/Images/ab16898-25781-anti-n-myc-antibody-ncm-ii-100-chip-grade-flow-cytometry.jpg)
Flow Cytometry - Anti-n-Myc/MYCN antibody [NCM II 100] (ab16898)Overlay histogram showing SH-SY5Y cells stained with ab16898 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16898, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
This image was generated using the ascites version of the product.
