Anti-IL-33 antibody [002] - BSA and Azide free (ab277144)
![Anti-IL-33 antibody [002] - BSA and Azide free (ab277144)](https://www.abcam.com/ps/products/277/ab277144/Images/ab277144-2-AntiIL33-antibody-ICC-HeLa-cells.jpg "Anti-IL-33 antibody [002] - BSA and Azide free (ab277144)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [002] to IL-33 - BSA and Azide free
- Suitable for: ICC, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-IL-33 antibody [002] - BSA and Azide free
See all IL-33 primary antibodies -
Description
Rabbit monoclonal [002] to IL-33 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC Human
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Immunogen
Recombinant fragment corresponding to Human IL-33 aa 112-270. Genbank: NP_254274.1
Database link: O95760 -
Positive control
- Flow cyt: HUVEC cells. ICC: HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Constituent: 100% PBS
0.2 µm filtered solution -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
002 -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry - Anti-IL-33 antibody (ab277144)
Immunofluorescence staining of IL-33 in HeLa (human epithelial cell line from cervix adenocarcinoma) cells. Cells were fixed with 4% PFA, permeabilized with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with ab277144 at 1/60 dilution at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).
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Flow Cytometry - Anti-IL-33 antibody (ab277144)Flow cytometric analysis of Purified anti-Human IL-33 on HUVEC cells. HUVEC cells were stained with ab277144 at 1/25 dilution (Bold line), then stained with a FITC-conjugated second step antibody.
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![Anti-IL-33 antibody [002] - BSA and Azide free (ab277144)](https://www.abcam.com/ps/products/277/ab277144/Images/ab277144-3-benefits-of-recombinant-antibodies.png)
Anti-IL-33 antibody [002] - BSA and Azide free (ab277144)