All lanes : Anti-ABL1 (phospho Y393) antibody [19HCLC] (ab277782) at 2 µg/ml

Lane 1 : K562 (human chronic myelogenous leukemia lymphoblast cell line) whole cell extract
Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extract
Lane 3 : KARPAS 299 whole cell extract
Lane 4 : Raji whole cell extract
Lane 5 : Ramos (human Burkitt's lymphoma cell line) whole cell extract
Lane 6 : A549 (human lung carcinoma cell line) whole cell extract
Lane 7 : MDA-MB-231 (human breast adenocarcinoma cell line) whole cell extract
Lane 8 : U-2 OS (human bone osteosarcoma epithelial cell line) whole cell extract

Developed using the ECL technique.

Predicted band size: 122 kDa

For immunofluorescence analysis K-562 cells were fixed and permeabilized for detection of endogenous CAbl pY393 Isoform A using Anti-CAbl pY393 Isoform A Recombinant Rabbit Multiclonal Antibody (ab277782, 2 μ/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal^™ Secondary Antibody, Alexa Fluor^® 488 conjugate (1/2000). Panel a) shows representative cells that were stained for detection and localization of CAbl pY393 protein (green), Panel b) is stained for nuclei (blue) using SlowFade^® Gold Antifade Mountant with DAPI. Panel c) represents cytoskeletal F-actin staining using Rhodamine Phalloidin (1/300). Panel d) is a composite image of Panels a, b and c clearly demonstrating cytoplasmic localization of CAbl pY393. Panel e) shows loss of signal by competition with the CAblpY393 peptide demonstrating antibody specificity, and panel f) demonstrates no competition with the non-phospho peptide. Panel μ) shows untreated cells with no signal. The images were captured at 60X magnification.