Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-n-Myc/MYCN antibody [EPR18982-8R-3] - ChIP Grade (ab227822)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18982-8R-3] to n-Myc/MYCN - ChIP Grade
  • Suitable for: ChIP, WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-n-Myc/MYCN antibody [EPR18982-8R-3] - ChIP Grade
    See all n-Myc/MYCN primary antibodies

  • Description

    Rabbit monoclonal [EPR18982-8R-3] to n-Myc/MYCN - ChIP Grade

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ChIP
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: IMR-32 whole cell lysate. IP: IMR-32 whole cell lysate. ChIP: Chromatin prepared from IMR-32 cells.

  • General notes

     This product was previously labelled as n-Myc

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • ChIP - Anti-n-Myc/MYCN antibody [EPR18982-8R-3] - ChIP Grade (ab227822)
    ChIP - Anti-n-Myc/MYCN antibody [EPR18982-8R-3] - ChIP Grade (ab227822)

    Chromatin was prepared from IMR-32 cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The ChIP was performed with 25 µg of chromatin, 5 µg of ab227822 (red), and 20 µl of Protein A/G sepharose beads. 5 μg of rabbit normal IgG was added to the beads control (gray). The immunoprecipitated DNA was quantified by real time PCR (SYBR green approach). Primers and probes are located in the first kb of the transcribed region.

  • Western blot - Anti-n-Myc/MYCN antibody [EPR18982-8R-3] - ChIP Grade (ab227822)
    Western blot - Anti-n-Myc/MYCN antibody [EPR18982-8R-3] - ChIP Grade (ab227822)
    All lanes : Anti-n-Myc/MYCN antibody [EPR18982-8R-3] - ChIP Grade (ab227822) at 1/1000 dilution

    Lane 1 : IMR-32 (human neuroblastoma neuroblast cell line) whole cell lysate
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 50 kDa
    Observed band size: 62 kDa
    why is the actual band size different from the predicted?


    Exposure time: 103 seconds


    Blocking and dilution buffer: 5% NFDM/TBST 

    The expression profile observed is consistent with what has been described in the literature (PMID: 11034201; PMID: 27197171; PMID: 23792191).

    Negative control: HeLa (PMID: 27197171).

  • Immunoprecipitation - Anti-n-Myc/MYCN antibody [EPR18982-8R-3] - ChIP Grade (ab227822)
    Immunoprecipitation - Anti-n-Myc/MYCN antibody [EPR18982-8R-3] - ChIP Grade (ab227822)

    n-Myc/MYCN was immunoprecipitated from 0.35 mg of IMR-32 (human neuroblastoma neuroblast cell line) whole cell lysate with ab227822 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab227822 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1: IMR-32 whole cell lysate 10 μg (Input).

    Lane 2: ab227822 IP in IMR-32 whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab227822 in IMR-32 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 3 seconds.

    The expression profile observed is consistent with what has been described in the literature (PMID: 17938259; PMID: 2657399).

  • Anti-n-Myc/MYCN antibody [EPR18982-8R-3] - ChIP Grade (ab227822)
    Anti-n-Myc/MYCN antibody [EPR18982-8R-3] - ChIP Grade (ab227822)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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