Anti-n-Myc/MYCN antibody [EPR18982-8R-3] - ChIP Grade (ab227822)
![Anti-n-Myc/MYCN antibody [EPR18982-8R-3] - ChIP Grade (ab227822)](https://www.abcam.com/ps/products/227/ab227822/Images/ab227822-5-AntinMycMYCN-antibody-EPR189828R3ChIP.jpg "Anti-n-Myc/MYCN antibody [EPR18982-8R-3] - ChIP Grade (ab227822)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18982-8R-3] to n-Myc/MYCN - ChIP Grade
- Suitable for: ChIP, WB, IP
- Reacts with: Human
Overview
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Product name
Anti-n-Myc/MYCN antibody [EPR18982-8R-3] - ChIP Grade
See all n-Myc/MYCN primary antibodies -
Description
Rabbit monoclonal [EPR18982-8R-3] to n-Myc/MYCN - ChIP Grade -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ChIP HumanIP HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: IMR-32 whole cell lysate. IP: IMR-32 whole cell lysate. ChIP: Chromatin prepared from IMR-32 cells.
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General notes
This product was previously labelled as n-Myc
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 49% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18982-8R-3 -
Isotype
IgG -
Research areas
Images
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ChIP - Anti-n-Myc/MYCN antibody [EPR18982-8R-3] - ChIP Grade (ab227822)
Chromatin was prepared from IMR-32 cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The ChIP was performed with 25 µg of chromatin, 5 µg of ab227822 (red), and 20 µl of Protein A/G sepharose beads. 5 μg of rabbit normal IgG was added to the beads control (gray). The immunoprecipitated DNA was quantified by real time PCR (SYBR green approach). Primers and probes are located in the first kb of the transcribed region.
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![Western blot - Anti-n-Myc/MYCN antibody [EPR18982-8R-3] - ChIP Grade (ab227822)](https://www.abcam.com/ps/products/227/ab227822/Images/ab227822-312399-anti-n-myc-antibody-epr18982-8r-3-chip-grade-western-blot.jpg)
Western blot - Anti-n-Myc/MYCN antibody [EPR18982-8R-3] - ChIP Grade (ab227822)All lanes : Anti-n-Myc/MYCN antibody [EPR18982-8R-3] - ChIP Grade (ab227822) at 1/1000 dilution
Lane 1 : IMR-32 (human neuroblastoma neuroblast cell line) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 50 kDa
Observed band size: 62 kDa why is the actual band size different from the predicted?
Exposure time: 103 secondsBlocking and dilution buffer: 5% NFDM/TBST
The expression profile observed is consistent with what has been described in the literature (PMID: 11034201; PMID: 27197171; PMID: 23792191).
Negative control: HeLa (PMID: 27197171).
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![Immunoprecipitation - Anti-n-Myc/MYCN antibody [EPR18982-8R-3] - ChIP Grade (ab227822)](https://www.abcam.com/ps/products/227/ab227822/Images/ab227822-312400-anti-n-myc-antibody-epr18982-8r-3-chip-grade-immunoprecipitation.jpg)
Immunoprecipitation - Anti-n-Myc/MYCN antibody [EPR18982-8R-3] - ChIP Grade (ab227822)n-Myc/MYCN was immunoprecipitated from 0.35 mg of IMR-32 (human neuroblastoma neuroblast cell line) whole cell lysate with ab227822 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab227822 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Lane 1: IMR-32 whole cell lysate 10 μg (Input).
Lane 2: ab227822 IP in IMR-32 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab227822 in IMR-32 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds.The expression profile observed is consistent with what has been described in the literature (PMID: 17938259; PMID: 2657399).
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![Anti-n-Myc/MYCN antibody [EPR18982-8R-3] - ChIP Grade (ab227822)](https://www.abcam.com/ps/products/227/ab227822/Images/ab227822-6-benefits-of-recombinant-antibodies.png)
Anti-n-Myc/MYCN antibody [EPR18982-8R-3] - ChIP Grade (ab227822)
