Anti-CREB (phospho S121) antibody (ab60941)
Key features and details
- Rabbit polyclonal to CREB (phospho S121)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CREB (phospho S121) antibody
See all CREB primary antibodies -
Description
Rabbit polyclonal to CREB (phospho S121) -
Host species
Rabbit -
Specificity
Detects endogenous levels of CREB only when phosphorylated at serine 121. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic phosphopeptide derived from human CREB around the phosphorylation site of serine 121 (T-D-SP-Q-K).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CREB (phospho S121) antibody (ab60941)All lanes : Anti-CREB (phospho S121) antibody (ab60941) at 1/500 dilution
Lane 1 : HeLa cell extracts treated
with Etoposide (25uM, 24hours)
Lane 2 : HeLa cell extracts treated
with Etoposide (25uM, 24hours) with immunizing peptide
Predicted band size: 37 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?
