Anti-n-Myc/MYCN antibody (ab24193)
Key features and details
- Rabbit polyclonal to n-Myc/MYCN
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-n-Myc/MYCN antibody
See all n-Myc/MYCN primary antibodies -
Description
Rabbit polyclonal to n-Myc/MYCN -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Non human primates
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Immunogen
Synthetic peptide corresponding to Human n-Myc/MYCN aa 1-100 conjugated to keyhole limpet haemocyanin. Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human n-Myc/MYCN.
Read Abcam's proprietary immunogen policy (Peptide available as ab31595.)
Database link: P04198
(Peptide available asab31595, ab31596) -
General notes
This product was previously labelled as n-Myc
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab24193 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa). Target
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Function
May function as a transcription factor. -
Involvement in disease
Note=Amplification of the N-MYC gene is associated with a variety of human tumors, most frequently neuroblastoma, where the level of amplification appears to increase as the tumor progresses.
Defects in MYCN are the cause of microcephaly-oculo-digito-esophageal-duodenal syndrome (MODED) [MIM:164280]; also known as oculodigitoesophagoduodenal syndrome (ODED). Microcephaly-oculo-digito-esophageal-duodenal syndrome is characterized by variable combinations of esophageal and duodenal atresias, microcephaly, learning disability and limb malformations. Cardiac and renal malformations, vertebral anomalies, and deafness have also been described.
Defects in MYCN are the cause of microcephaly and digital abnormalities with normal intelligence (MCPHDANI) [MIM:602585]. -
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain. -
Developmental stage
Expressed during fetal development. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 4613 Human
- Entrez Gene: 18109 Mouse
- Entrez Gene: 298894 Rat
- Omim: 164840 Human
- SwissProt: P18444 Chicken
- SwissProt: P04198 Human
- SwissProt: P03966 Mouse
- SwissProt: Q63379 Rat
see all -
Alternative names
- bHLHe37 antibody
- Class E basic helix-loop-helix protein 37 antibody
- MODED antibody
see all
Images
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Western blot - Anti-n-Myc/MYCN antibody (ab24193)All lanes : Anti-n-Myc/MYCN antibody (ab24193) at 1 µg/ml
Lane 1 : Heart (Human) Whole Cell Lysate - fetal normal tissue
Lane 2 : Heart (Mouse) Tissue Lysate
Lane 3 : Skeletal Muscle (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG VHH Single Domain (HRP) (ab191866) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 49 kDa
Observed band size: 49 kDa
Additional bands at: 190 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 secondsThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab24193 overnight at 4°C. Antibody binding was detected using an anti-rabbit IgG VHH single domain antibody conjugated to HRP (ab191866), and visualised using ECL development solution ab133406.
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Western blot - Anti-n-Myc/MYCN antibody (ab24193)All lanes : Anti-n-Myc/MYCN antibody (ab24193) at 1 µg/ml
Lane 1 : Heart (Human) Whole Cell Lysate - fetal normal tissue (ab29432)
Lane 2 : Heart (Human) Nuclear Lysate - fetal normal tissue (ab29428)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 49 kDa
Observed band size: 49 kDa
Additional bands at: 25 kDa (possible cleavage fragment), 25 kDa (possible cross reactivity), 33 kDa (possible cleavage fragment), 33 kDa (possible cross reactivity), 39 kDa (possible cleavage fragment), 39 kDa (possible cross reactivity)
Protocols
References (11)
ab24193 has been referenced in 11 publications.
- Wang XH et al. FGF represses metastasis of neuroblastoma regulated by MYCN and TGF-ß1 induced LMO1 via control of let-7 expression. Brain Res 1704:219-228 (2019). PubMed: 30321496
- Zhu S et al. BMP4 and Neuregulin regulate the direction of mouse neural crest cell differentiation. Exp Ther Med 17:3883-3890 (2019). PubMed: 31007733
- Deng J et al. ß2-adrenergic receptor signaling promotes neuroblastoma cell proliferation by activating autophagy. Oncol Rep N/A:N/A (2019). PubMed: 31524241
- Wang Y et al. Downregulation of N-Myc inhibits neuroblastoma cell growth via the Wnt/ß-catenin signaling pathway. Mol Med Rep 18:377-384 (2018). PubMed: 29749516
- Jiang X et al. Huwe1 is a novel mediator of protection of neural progenitor L2.3 cells against oxygen-glucose deprivation injury. Mol Med Rep 18:4595-4602 (2018). PubMed: 30221657
Images
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Western blot - Anti-n-Myc/MYCN antibody (ab24193)All lanes : Anti-n-Myc/MYCN antibody (ab24193) at 1 µg/ml
Lane 1 : Heart (Human) Whole Cell Lysate - fetal normal tissue
Lane 2 : Heart (Mouse) Tissue Lysate
Lane 3 : Skeletal Muscle (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG VHH Single Domain (HRP) (ab191866) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 49 kDa
Observed band size: 49 kDa
Additional bands at: 190 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 secondsThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab24193 overnight at 4°C. Antibody binding was detected using an anti-rabbit IgG VHH single domain antibody conjugated to HRP (ab191866), and visualised using ECL development solution ab133406.
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Western blot - Anti-n-Myc/MYCN antibody (ab24193)All lanes : Anti-n-Myc/MYCN antibody (ab24193) at 1 µg/ml
Lane 1 : Heart (Human) Whole Cell Lysate - fetal normal tissue (ab29432)
Lane 2 : Heart (Human) Nuclear Lysate - fetal normal tissue (ab29428)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 49 kDa
Observed band size: 49 kDa
Additional bands at: 25 kDa (possible cleavage fragment), 25 kDa (possible cross reactivity), 33 kDa (possible cleavage fragment), 33 kDa (possible cross reactivity), 39 kDa (possible cleavage fragment), 39 kDa (possible cross reactivity)
