Anti-RING1 antibody (ab32644)
Key features and details
- Rabbit polyclonal to RING1
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RING1 antibody
See all RING1 primary antibodies -
Description
Rabbit polyclonal to RING1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human RING1.
Read Abcam's proprietary immunogen policy (Peptide available as ab32805.) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab32644 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 42,39 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 42,39 kDa).Target
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Function
Constitutes one of the E3 ubiquitin-protein ligases that mediate monoubiquitination of 'Lys-119' of histone H2A, thereby playing a central role in histone code and gene regulation. H2A 'Lys-119' ubiquitination gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. Essential component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex act via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. Compared to RNF2/RING2, it does not have the main E3 ubiquitin ligase activity on histone H2A, and it may rather act as a modulator of RNF2/RING2 activity. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Contains 1 RING-type zinc finger. -
Cellular localization
Nucleus speckle. - Information by UniProt
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Database links
- Entrez Gene: 6015 Human
- Entrez Gene: 19763 Mouse
- Entrez Gene: 309626 Rat
- Omim: 602045 Human
- SwissProt: Q06587 Human
- SwissProt: O35730 Mouse
- SwissProt: Q6MGB6 Rat
- Unigene: 727565 Human
see all -
Alternative names
- Ring1A antibody
- E3 ubiquitin-protein ligase RING1 antibody
- Polycomb complex protein RING1 antibody
see all
Images
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Western blot - Anti-RING1 antibody (ab32644)
Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: RING1 knockout HAP1 whole cell lysate (20 µg)
Lane 3: MOLT4 whole cell lysate (20 µg)
Lane 4: LnCaP whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab32644 observed at 55 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab32644 was shown to recognize RING1 in wild type cells as signal was lost at the expected MW in RING1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and RING1 knockout samples were subjected to SDS-PAGE. ab32644 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 2 µg/ml and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab32644 has been referenced in 7 publications.
- Ahmed KM et al. ß1-Integrin Impacts Rad51 Stability and DNA Double-Strand Break Repair by Homologous Recombination. Mol Cell Biol 38:N/A (2018). PubMed: 29463647
- Wang J et al. Asymmetric Expression of LincGET Biases Cell Fate in Two-Cell Mouse Embryos. Cell 175:1887-1901.e18 (2018). PubMed: 30550787
- Sun D et al. LncRNA GAS5 inhibits microglial M2 polarization and exacerbates demyelination. EMBO Rep N/A:N/A (2017). PubMed: 28808113
- Palau A et al. Polycomb protein RING1A limits hematopoietic differentiation in myelodysplastic syndromes. Oncotarget 8:115002-115017 (2017). PubMed: 29383137
- Koo JW et al. Epigenetic basis of opiate suppression of Bdnf gene expression in the ventral tegmental area. Nat Neurosci 18:415-22 (2015). ChIP ; Rat . PubMed: 25643298
- Basu A et al. YY1 DNA binding and interaction with YAF2 is essential for Polycomb recruitment. Nucleic Acids Res N/A:N/A (2013). ChIP ; Human . PubMed: 24285299
- Argiropoulos B et al. Linkage of the potent leukemogenic activity of Meis1 to cell-cycle entry and transcriptional regulation of cyclin D3. Blood 115:4071-82 (2010). ChIP ; Mouse . PubMed: 20237320
Images
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Western blot - Anti-RING1 antibody (ab32644)
Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: RING1 knockout HAP1 whole cell lysate (20 µg)
Lane 3: MOLT4 whole cell lysate (20 µg)
Lane 4: LnCaP whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab32644 observed at 55 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab32644 was shown to recognize RING1 in wild type cells as signal was lost at the expected MW in RING1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and RING1 knockout samples were subjected to SDS-PAGE. ab32644 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 2 µg/ml and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
