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Epigenetics and Nuclear Signaling Chromatin Remodeling Polycomb Silencing PRC1

Anti-RING1 antibody [8E8A2] (ab181756)

Anti-RING1 antibody [8E8A2] (ab181756)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [8E8A2] to RING1
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-RING1 antibody [8E8A2]
    See all RING1 primary antibodies
  • Description

    Mouse monoclonal [8E8A2] to RING1
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment corresponding to Human RING1 aa 79-263. Expressed in E. Coli.
    Sequence:

    SGNKECPTCRKKLVSKRSLRPDPNFDALISKIYPSREEYEAHQDRVLIRL SRLHNQQALSSSIEEGLRMQAMHRAQRVRRPIPGSDQTTTMSGGEGEPGE GEGDGEDVSSDSAPDSAPGPAPKRPRGGGAGGSSVGTGGGGTGGVGGGAG SEDSGDRGGTLGGGTLGPPSPPGAPSPPEPGGEIE


    Database link: Q06587
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • RING1 recombinant protein; RING1 (aa 79-263)-hIgGFc transfected HEK293 cell lysate; A431 and HeLa cells.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: 99% PBS

    Contains 0.5% protein stabilizer.
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from tissue culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    8E8A2
  • Isotype

    IgG1
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Remodeling
    • Polycomb Silencing
    • PRC1

Images

  • Western blot - Anti-RING1 antibody [8E8A2] (ab181756)
    Western blot - Anti-RING1 antibody [8E8A2] (ab181756)
    All lanes : Anti-RING1 antibody [8E8A2] (ab181756) at 1/500 dilution

    Lane 1 : HEK293 cell lysate, non-transfected
    Lane 2 : HEK293 cell lysate, transfected with RING1 (aa 79-263)-hIgGFc

    Predicted band size: 42 kDa

  • Western blot - Anti-RING1 antibody [8E8A2] (ab181756)
    Western blot - Anti-RING1 antibody [8E8A2] (ab181756)
    Anti-RING1 antibody [8E8A2] (ab181756) at 1/500 dilution + Human RING1 recombinant protein

    Predicted band size: 42 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-RING1 antibody [8E8A2] (ab181756)
    Immunocytochemistry/ Immunofluorescence - Anti-RING1 antibody [8E8A2] (ab181756)

    Immunofluorescent analysis of HeLa cells labeling RING1 with ab181756 at 1/200 dilution (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

  • Immunocytochemistry/ Immunofluorescence - Anti-RING1 antibody [8E8A2] (ab181756)
    Immunocytochemistry/ Immunofluorescence - Anti-RING1 antibody [8E8A2] (ab181756)

    Immunofluorescent analysis of A431 cells labeling RING1 with ab181756 at 1/200 dilution (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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