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Epigenetics and Nuclear Signaling Chromatin Remodeling Polycomb Silencing PRC1

Anti-RING1 antibody [8C12F4] (ab175149)

Anti-RING1 antibody [8C12F4] (ab175149)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [8C12F4] to RING1
  • Suitable for: WB, ELISA, Flow Cyt, IHC-P
  • Knockout validated
  • Reacts with: Human, Recombinant fragment
  • Isotype: IgG1

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Overview

  • Product name

    Anti-RING1 antibody [8C12F4]
    See all RING1 primary antibodies
  • Description

    Mouse monoclonal [8C12F4] to RING1
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    Recombinant fragment
    See all applications and species data
  • Immunogen

    Recombinant fragment corresponding to Human RING1 aa 79-263.
    Database link: Q06587

  • Positive control

    • RING1 recombinant protein; RING1 (aa: 79-263)-hIgGFc transfected HEK293 cell lysate; MOLT4, LNCaP, Hela, HEK-293 and Jurkat cell lysates; Human cervical cancer and Human rectum cancer tissues; HeLa cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: 99% PBS

    0.5% protein stabilizer
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from tissue culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    8C12F4
  • Isotype

    IgG1
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Remodeling
    • Polycomb Silencing
    • PRC1

Images

  • Western blot - Anti-RING1 antibody [8C12F4] (ab175149)
    Western blot - Anti-RING1 antibody [8C12F4] (ab175149)

    Lane 1: Wild-type HAP1 cell lysate (40 µg)
    Lane 2: RING1 knockout HAP1 cell lysate (40 µg)
    Lane 3: MOLT4 cell lysate (20 µg)
    Lane 4: LnCaP cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab175149 observed at 50 kDa. Red - loading control, ab181602, observed at 37 kDa.

    ab175149 was shown to specifically react with RING1 when RING1 knockout samples were used. Wild-type and RING1 knockout samples were subjected to SDS-PAGE. Ab175149 and ab181602 (loading control to GAPDH) were diluted at 1/500 and 1/10000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-RING1 antibody [8C12F4] (ab175149)
    Western blot - Anti-RING1 antibody [8C12F4] (ab175149)
    Anti-RING1 antibody [8C12F4] (ab175149) at 1/500 dilution + RING1 recombinant protein

    Predicted band size: 42 kDa

  • Western blot - Anti-RING1 antibody [8C12F4] (ab175149)
    Western blot - Anti-RING1 antibody [8C12F4] (ab175149)
    All lanes : Anti-RING1 antibody [8C12F4] (ab175149) at 1/500 dilution

    Lane 1 : HEK293 cell lysate
    Lane 2 : RING1 (aa: 79-263)-hIgGFc transfected HEK293 cell lysate

    Predicted band size: 42 kDa

  • Western blot - Anti-RING1 antibody [8C12F4] (ab175149)
    Western blot - Anti-RING1 antibody [8C12F4] (ab175149)
    All lanes : Anti-RING1 antibody [8C12F4] (ab175149) at 1/500 dilution

    Lane 1 : MOLT4 cell lysate
    Lane 2 : LNCaP cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : HEK293 cell lysate
    Lane 5 : Jurkat cell lysate

    Predicted band size: 42 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RING1 antibody [8C12F4] (ab175149)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RING1 antibody [8C12F4] (ab175149)

    Immunohistochemical analysis of paraffin embedded Human rectum carcinoma tissue labeling RING1 with ab175149 at 1/200 followed by DAB staining.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RING1 antibody [8C12F4] (ab175149)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RING1 antibody [8C12F4] (ab175149)

    Immunohistochemical analysis of paraffin embedded Human cervical carcinoma tissue labeling RING1 with ab175149 at 1/200 followed by DAB staining.

  • Flow Cytometry - Anti-RING1 antibody [8C12F4] (ab175149)
    Flow Cytometry - Anti-RING1 antibody [8C12F4] (ab175149)

    Flow Cytometrical analysis of HeLa cells labeling RING1 with ab175149 at 1/200 (green) compared to a negative control (red).

  • ELISA - Anti-RING1 antibody [8C12F4] (ab175149)
    ELISA - Anti-RING1 antibody [8C12F4] (ab175149)

    ELISA using ab175149 at 1/10000.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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