Human RNF2 (RING2 / RING1B) knockout HeLa cell lysate (ab257640)
Properties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit ab260311 - Human RNF2 knockout HeLa cell lysate 1 x 100µg ab255552 - Human wild-type HeLa cell lysate 1 x 100µg -
Research areas
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Cell type
epithelial -
Disease
Adenocarcinoma -
Gender
Female -
STR Analysis
Amelogenin X D5S818: 11, 12 D13S317: 12, 13.3 D7S820: 8, 12 D16S539: 9, 10 vWA: 16, 18 TH01: 7 TPOX: 8, 12 CSF1PO: 9, 10
Images
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Western blot - Human RNF2 (RING2 / RING1B) knockout HeLa cell lysate (ab257640)Lane 1: Wild-type HeLa cell lysate (20µg)
Lane 2: RNF2 knockout HeLa cell lysate (20µg)
Lanes 1- 2: Merged signal (red and green). Green - ab181140 observed at 42 kDa. Red - loading control, ab7291 observed at 50 kDa.
ab181140 Anti-RING2 / RING1B / RNF2 antibody [EPR12245] was shown to specifically react with RING2 / RING1B / RNF2 in wild-type HeLa cells in western blot. The band observed in the knockout cell line ab264845 (knockout cell lysate ab257640) lane below 42kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type and RING2 / RING1B / RNF2 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab181140 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging. -
Sanger Sequencing - Human RNF2 knockout HeLa cell lysate (ab257640)Allele-1: 8 bp deletion in exon 2
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Sanger Sequencing - Human RNF2 knockout HeLa cell lysate (ab257640)Allele-2: Insertion of the selection cassette in exon 2