Anti-SCMH1 antibody [OTI5E6] (ab236411)
Key features and details
- Mouse monoclonal [OTI5E6] to SCMH1
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-SCMH1 antibody [OTI5E6] -
Description
Mouse monoclonal [OTI5E6] to SCMH1 -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human SCMH1 aa 1-350. (NP_036368) produced in E.coli.
Database link: Q96GD3 -
Positive control
- WB: HEK-293T transfected with pCMV6-ENTRY SCMH1, whole cell lysate. IHC-P: Human breast adenocarcinoma, colon adenocarcinoma, pancreatic carcinoma, prostate carcinoma and bladder tissue.
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General notes
Clone OTI5E6 (formerly 5E6).
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
Purified from cell culture supernatant. -
Clonality
Monoclonal -
Clone number
OTI5E6 -
Isotype
IgG2b -
Research areas
Images
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All lanes : Anti-SCMH1 antibody [OTI5E6] (ab236411) at 1/2000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with pCMV6-ENTRY control, whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with pCMV6-ENTRY SCMH1, whole cell lysate
Lysates/proteins at 5 µg per lane.
Predicted band size: 73 kDa
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Paraffin-embedded human breast adenocarcinoma tissue stained for SCMH1 using ab236411 at 1/150 dilution in immunohistochemical analysis.
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Paraffin-embedded human colon adenocarcinoma tissue stained for SCMH1 using ab236411 at 1/150 dilution in immunohistochemical analysis.
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Paraffin-embedded human pancreatic carcinoma tissue stained for SCMH1 using ab236411 at 1/150 dilution in immunohistochemical analysis.
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Paraffin-embedded human prostate carcinoma tissue stained for SCMH1 using ab236411 at 1/150 dilution in immunohistochemical analysis.
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Paraffin-embedded human bladder tissue stained for SCMH1 using ab236411 at 1/150 dilution in immunohistochemical analysis.