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Epigenetics and Nuclear Signaling Chromatin Remodeling Polycomb Silencing PRC1

Anti-EDR1 / PHC1 antibody [1F3F3] (ab175424)

Anti-EDR1 / PHC1 antibody [1F3F3] (ab175424)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [1F3F3] to EDR1 / PHC1
  • Suitable for: WB, Flow Cyt
  • Reacts with: Human, Recombinant fragment
  • Isotype: IgG2b

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Overview

  • Product name

    Anti-EDR1 / PHC1 antibody [1F3F3]
  • Description

    Mouse monoclonal [1F3F3] to EDR1 / PHC1
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Human, Recombinant fragment
    Predicted to work with: Mouse
  • Immunogen

    Recombinant fragment corresponding to Human EDR1/ PHC1 aa 758-1004. Expressed in E. Coli.
    Sequence:

    LKESEKPLQTGLPTGLTENQSGGPLGVDSPSAELDKKANLLKCEYCGKYA PAEQFRGSKRFCSMTCAKRYNVSCSHQFRLKRKKMKEFQEANYARVRRRG PRRSSSDIARAKIQGKCHRGQEDSSRGSDNSSYDEALSPTSPGPLSVRAG HGERDLGNPNTAPPTPELHGINPVFLSSNPSRWSVEEVYEFIASLQGCQE IAEEFRSQEIDGQALLLLKEEHLMSAMNIKLGPALKICAKINVLKET


    Database link: P78364
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • EDR1 / PHC1 recombinant protein; HEK293 cells.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: 99% PBS

    0.5% protein stabilizer
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from tissue culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    1F3F3
  • Isotype

    IgG2b
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Remodeling
    • Polycomb Silencing
    • PRC1
    • Epigenetics and Nuclear Signaling
    • Chromatin Remodeling
    • Polycomb Silencing
    • PRC2
    • Stem Cells
    • Hematopoietic Progenitors
    • Intracellular Molecules

Images

  • Western blot - Anti-EDR1 / PHC1 antibody [1F3F3] (ab175424)
    Western blot - Anti-EDR1 / PHC1 antibody [1F3F3] (ab175424)
    All lanes : Anti-EDR1 / PHC1 antibody [1F3F3] (ab175424) at 1/500 dilution

    Lane 1 : HEK293 cell lysate
    Lane 2 : EDR1/PHC1 (amino acids 758-1004)-hIgGFc transfected HEK293 cell lysate

    Predicted band size: 106 kDa

  • Western blot - Anti-EDR1 / PHC1 antibody [1F3F3] (ab175424)
    Western blot - Anti-EDR1 / PHC1 antibody [1F3F3] (ab175424)
    Anti-EDR1 / PHC1 antibody [1F3F3] (ab175424) at 1/500 dilution + EDR1/PHC1 recombinant protein (amino acids 758-1004).

    Predicted band size: 106 kDa

  • Flow Cytometry - Anti-EDR1 / PHC1 antibody [1F3F3] (ab175424)
    Flow Cytometry - Anti-EDR1 / PHC1 antibody [1F3F3] (ab175424)

    Flow cytometric analysis of HEK293 cells labeling EDR1 / PHC1 with ab175424 at 1/200 dilution (green) and negative control (red).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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    Applications: ICC/IF, IHC-P

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