Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-RING2 / RING1B / RNF2 (phospho S168) antibody [EPR20902-58] (ab234421)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20902-58] to RING2 / RING1B / RNF2 (phospho S168)
  • Suitable for: WB, IP, Dot blot
  • Knockout validated
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-RING2 / RING1B / RNF2 (phospho S168) antibody [EPR20902-58]
    See all RING2 / RING1B / RNF2 primary antibodies

  • Description

    Rabbit monoclonal [EPR20902-58] to RING2 / RING1B / RNF2 (phospho S168)

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Mouse
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Overexpression of wild type RNF2 in RNF2 knock out mouse embryonic stem cells derived from C57BL/6 mouse, whole cell lysate; NCCIT, HEK-293T, HepG2 and F9 whole cell lysates; mouse testis tissue lysate. Dot blot: RNF2 (phospho S168) peptide (aa161-172) and RNF2 (phospho S168) peptide (aa165-176). IP: F9 whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR20902-58

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-RING2 / RING1B / RNF2 (phospho S168) antibody [EPR20902-58] (ab234421)
    Western blot - Anti-RING2 / RING1B / RNF2 (phospho S168) antibody [EPR20902-58] (ab234421)
    All lanes : Anti-RING2 / RING1B / RNF2 (phospho S168) antibody [EPR20902-58] (ab234421) at 1/1000 dilution

    Lane 1 : Overexpression of wild type RNF2 in RNF2 knock out mouse embryonic stem cells derived from C57BL/6 mouse, whole cell lysate
    Lane 2 : Overexpression of S168A mutant RNF2 in RNF2 knock out mouse embryonic stem cells derived from C57BL/6 mouse, whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 38 kDa
    Observed band size: 40 kDa
    why is the actual band size different from the predicted?



    Blocking and dilution buffer:  5% NFDM/TBST.

    Lysates were kindly provided by Dr Jinzhong Qin.

  • Dot Blot - Anti-RING2 / RING1B / RNF2 (phospho S168) antibody [EPR20902-58] (ab234421)
    Dot Blot - Anti-RING2 / RING1B / RNF2 (phospho S168) antibody [EPR20902-58] (ab234421)

    Dot blot analysis of RING2 / RING1B / RNF2 (phospho S168) peptide labeled with ab234421 at 1/1000 dilution.

    Lane 1: RING2 / RING1B / RNF2 (phospho S168) peptide (aa161-172).
    Lane 2: RING2 / RING1B / RNF2 (phospho S168) peptide (aa165-176).
    Lane 3: RING2 / RING1B / RNF2 non-phospho peptide (aa161-172).

    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Immunoprecipitation - Anti-RING2 / RING1B / RNF2 (phospho S168) antibody [EPR20902-58] (ab234421)
    Immunoprecipitation - Anti-RING2 / RING1B / RNF2 (phospho S168) antibody [EPR20902-58] (ab234421)

    RING2 / RING1B / RNF2 (phospho S168) was immunoprecipitated from 0.35 mg of F9 (mouse embryonic testicular cancer cell line) whole cell lysate with a234421 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab234421 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used at 1/5000 dilution.

    Lane 1: F9 whole cell lysate 10 μg (Input).
    Lane 2: ab234421 IP in F9 whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab234421 in F9 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 30 seconds.

  • Western blot - Anti-RING2 / RING1B / RNF2 (phospho S168) antibody [EPR20902-58] (ab234421)
    Western blot - Anti-RING2 / RING1B / RNF2 (phospho S168) antibody [EPR20902-58] (ab234421)
    All lanes : Anti-RING2 / RING1B / RNF2 (phospho S168) antibody [EPR20902-58] (ab234421) at 1/1000 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : NCCIT (human pluripotent embryonic carcinoma cell line) whole cell lysate
    Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 5 : F9 (mouse embryonic testicular cancer cell line) whole cell lysate
    Lane 6 : Mouse testis tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 38 kDa
    Observed band size: 40 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times.

    Lane 1: 3 minutes; Lanes 2 & 3: 15 seconds; Lanes 4-6: 3 minutes.

  • Anti-RING2 / RING1B / RNF2 (phospho S168) antibody [EPR20902-58] (ab234421)
    Anti-RING2 / RING1B / RNF2 (phospho S168) antibody [EPR20902-58] (ab234421)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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