Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-RING2 / RING1B / RNF2 (phospho S168) antibody [EPR20902-58] - BSA and Azide free (ab256315)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20902-58] to RING2 / RING1B / RNF2 (phospho S168) - BSA and Azide free
  • Suitable for: WB, Dot blot, IP
  • Knockout validated
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-RING2 / RING1B / RNF2 (phospho S168) antibody [EPR20902-58] - BSA and Azide free
    See all RING2 / RING1B / RNF2 primary antibodies

  • Description

    Rabbit monoclonal [EPR20902-58] to RING2 / RING1B / RNF2 (phospho S168) - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Mouse
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Overexpression of wild type RNF2 in RNF2 knock out mouse embryonic stem cells derived from C57BL/6 mouse, whole cell lysate; NCCIT, HEK-293T, HepG2 and F9 whole cell lysates; mouse testis tissue lysate. Dot blot: RNF2 (phospho S168) peptide (aa161-172) and RNF2 (phospho S168) peptide (aa165-176). IP: F9 whole cell lysate.

  • General notes

    Ab256315 is the carrier-free version of ab234421. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab256315 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Dot Blot - Anti-RING2 / RING1B / RNF2 (phospho S168) antibody [EPR20902-58] - BSA and Azide free (ab256315)
    Dot Blot - Anti-RING2 / RING1B / RNF2 (phospho S168) antibody [EPR20902-58] - BSA and Azide free (ab256315)

    Dot blot analysis of RING2 / RING1B / RNF2 (phospho S168) peptide labeled with ab234421 at 1/1000 dilution.

    Lane 1: RING2 / RING1B / RNF2 (phospho S168) peptide (aa161-172).
    Lane 2: RING2 / RING1B / RNF2 (phospho S168) peptide (aa165-176).
    Lane 3: RING2 / RING1B / RNF2 non-phospho peptide (aa161-172).

    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab234421).

  • Immunoprecipitation - Anti-RING2 / RING1B / RNF2 (phospho S168) antibody [EPR20902-58] - BSA and Azide free (ab256315)
    Immunoprecipitation - Anti-RING2 / RING1B / RNF2 (phospho S168) antibody [EPR20902-58] - BSA and Azide free (ab256315)

    RING2 / RING1B / RNF2 (phospho S168) was immunoprecipitated from 0.35 mg of F9 (mouse embryonic testicular cancer cell line) whole cell lysate with a234421 at 1/30 dilution. Western blot was performed from the immunoprecipitate using abX234421 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used at 1/5000 dilution.

    Lane 1: F9 whole cell lysate 10 μg (Input).
    Lane 2: ab234421 IP in F9 whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab234421 in F9 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 30 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab234421).

  • Anti-RING2 / RING1B / RNF2 (phospho S168) antibody [EPR20902-58] - BSA and Azide free (ab256315)
    Anti-RING2 / RING1B / RNF2 (phospho S168) antibody [EPR20902-58] - BSA and Azide free (ab256315)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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