Salmon Sperm ssDNA (ab229278)
Overview
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Product name
Salmon Sperm ssDNA -
General notes
This product is a convenient, ready-to-use solution of salmon sperm DNA especially prepared for use in the preparation of pre-hybridization and hybridization solutions and as a DNA carrier in yeast transformation protocols and other related methods. This solution contains sheared single-stranded DNA molecules that can be used to block the non-specific attachment of probe DNA to the surface of a membrane (Southern) or to increase yeast transformation efficiency.
Background: Prepared by a modification of the method of Emanuel and Chaikoff, JBC 203, 164 (1953) from salmon testes DNA by mechanical shearing and heat denaturation to an average size of 100 to 200 base pairs. To reverse any renaturation occurring during storage, this material should be briefly boiled and rapidly chilled prior to use. >75% native nucleic acid.
Purity/Specifity: A260/A280: 1.85 % Hyperchromocity: 30.5.
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Tested applications
Suitable for: ChIP, In situ hybridization, Blockingmore details
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituents: 0.16% Tris HCl, 0.03% EDTA -
Concentration information loading... -
Research areas
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Relevance
The cell death process during apoptosis is essential for normal tissue homeostasis and cellular differentiation in multi cellular organisms. Single stranded DNA (ss DNA) is produced by nuclear fragmentation during the early stages of the apoptotic process. -
Cellular localization
Nuclear -
Alternative names
- Single stranded deoxyribose nucleic acid
- Single stranded DNA