Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-G3BP antibody [EPR13985(B)] - BSA and Azide free (ab240246)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13985(B)] to G3BP - BSA and Azide free
  • Suitable for: WB, Flow Cyt, ICC/IF, IP, IHC-P
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-G3BP antibody [EPR13985(B)] - BSA and Azide free
    See all G3BP primary antibodies

  • Description

    Rabbit monoclonal [EPR13985(B)] to G3BP - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cyt, ICC/IF, IP, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab240246 is the carrier-free version of ab181149. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab240246 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-G3BP antibody [EPR13985(B)] - BSA and Azide free (ab240246)
    Immunocytochemistry/ Immunofluorescence - Anti-G3BP antibody [EPR13985(B)] - BSA and Azide free (ab240246)

    Immunocytochemistry/Immunofluorescence analysis of Jurkat (Human T cell leukemia cell line from peripheral blood) labeling G3BP with Purified ab181149 at 1/500 dilution (5 µg/ml). Cells were fixed with 4% PFA and permeabilized with 0.1% tritonX-100. ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) at 1/1000 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181149).

  • Immunohistochemistry paraffin embedded sections - Anti-G3BP antibody [EPR13985(B)] - BSA and Azide free (ab240246)
    Immunohistochemistry paraffin embedded sections - Anti-G3BP antibody [EPR13985(B)] - BSA and Azide free (ab240246)

    Immunohistochemical analysis of paraffin-embedded Human muscle tissue staining G3BP using ab181149 at 1/100 dilution, and prediluted HRP Polymer for Rabbit IgG as a secondary antibody with Hematoxylin counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181149).

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry paraffin embedded sections - Anti-G3BP antibody [EPR13985(B)] - BSA and Azide free (ab240246)
    Immunohistochemistry paraffin embedded sections - Anti-G3BP antibody [EPR13985(B)] - BSA and Azide free (ab240246)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue staining G3BP using ab181149 at 1/100 dilution, and prediluted HRP Polymer for Rabbit IgG as a secondary antibody with Hematoxylin counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181149).

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-G3BP antibody [EPR13985(B)] - BSA and Azide free (ab240246)
    Immunoprecipitation - Anti-G3BP antibody [EPR13985(B)] - BSA and Azide free (ab240246)

    Western blot analysis of G3BP in immunoprecipitation pellets from Ramos cell lysate, using ab181149 at a 1/50 dilution. Anti-Rabbit IgG (HRP) specific to the non-reduced form of IgG was used as a secondary antibody at 1/1000 dilution. Blocking/dilution buffer and concentration: 5% NFDM/TBST

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181149).

  • Immunocytochemistry/ Immunofluorescence - Anti-G3BP antibody [EPR13985(B)] - BSA and Azide free (ab240246)
    Immunocytochemistry/ Immunofluorescence - Anti-G3BP antibody [EPR13985(B)] - BSA and Azide free (ab240246)

    Immunofluorescence analysis of 4% paraformaldehyde fixed 293 cells staining G3BP using ab181149 at 1/500 dilution (red), and Goat anti rabbit IgG (Alexa Fluor® 555) at 1/200 dilution as a secondary antibody. Dapi was used for counterstaining (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181149).

  • Flow Cytometry - Anti-G3BP antibody [EPR13985(B)] - BSA and Azide free (ab240246)
    Flow Cytometry - Anti-G3BP antibody [EPR13985(B)] - BSA and Azide free (ab240246)

    Flow cytometric analysis of 2% paraformaldehyde fixed Jurkat cells staining G3BP using ab181149 at 1/10 dilution, and FITC conjugated Goat anti rabbit IgG at 1/150 dilution as a secondary antibody (red curve). Rabbit monoclonal IgG was the negative control (green curve).

     

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181149).

  • Anti-G3BP antibody [EPR13985(B)] - BSA and Azide free (ab240246)
    Anti-G3BP antibody [EPR13985(B)] - BSA and Azide free (ab240246)

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