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Anti-REG4 antibody [EPR22810-327] - BSA and Azide free (ab256818)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22810-327] to REG4 - BSA and Azide free
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-REG4 antibody [EPR22810-327] - BSA and Azide free
    See all REG4 primary antibodies

  • Description

    Rabbit monoclonal [EPR22810-327] to REG4 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
    Unsuitable for: Flow Cyt or ICC/IF

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human colon and colon carcinoma tissue; IP: LoVo whole cell lysate.

  • General notes

    Ab256818 is the carrier-free version of ab255820. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab256818 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-REG4 antibody [EPR22810-327] - BSA and Azide free (ab256818)
    Immunoprecipitation - Anti-REG4 antibody [EPR22810-327] - BSA and Azide free (ab256818)

    REG4 was immunoprecipitated from 0.35 mg LoVo (human colorectal adenocarcinoma epithelial cell) whole cell lysate 10μg with ab255820 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab255820 1/1000 dilution (0.52 μg/ml). VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

    Lane 1: LoVo (human colorectal adenocarcinoma epithelial cell) whole cell lysate 10μg Lane 2: ab255820 IP in LoVo whole cell lysate
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab255820 in LoVo whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 3 min

    This blot was developed using a higher sensitivity ECL substrate.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255820).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REG4 antibody [EPR22810-327] - BSA and Azide free (ab256818)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REG4 antibody [EPR22810-327] - BSA and Azide free (ab256818)

    Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling REG4 with ab255820 at 1:2000 dilution (0.262 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human colon carcinoma (PMID: 25295732) The section was incubated with ab255820 for 15 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.


    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255820).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REG4 antibody [EPR22810-327] - BSA and Azide free (ab256818)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REG4 antibody [EPR22810-327] - BSA and Azide free (ab256818)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling REG4 with ab255820 at 1:2000 dilution (0.262 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human colon (PMID: 25295732) The section was incubated with ab255820 for 15 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.


    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255820).
  • Anti-REG4 antibody [EPR22810-327] - BSA and Azide free (ab256818)
    Anti-REG4 antibody [EPR22810-327] - BSA and Azide free (ab256818)

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