Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-REG4 antibody [EPR22810-327] (ab255820)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22810-327] to REG4
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-REG4 antibody [EPR22810-327]
    See all REG4 primary antibodies

  • Description

    Rabbit monoclonal [EPR22810-327] to REG4

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: LoVo whole cell lysates; IHC-P: Human colon and colon carcinoma tissue; IP: LoVo whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR22810-327

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-REG4 antibody [EPR22810-327] (ab255820)
    Western blot - Anti-REG4 antibody [EPR22810-327] (ab255820)
    All lanes : Anti-REG4 antibody [EPR22810-327] (ab255820) at 1/1000 dilution

    All lanes : LoVo (human colorectal adenocarcinoma epithelial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 18 kDa
    Observed band size: 14 kDa
    why is the actual band size different from the predicted?


    Exposure time: 59 seconds


    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 20126989).

    Negative control: HT-29 (PMID: 12455032).

    Blocking/diluting buffer and concentration: 5% NFDM/TBST
     

     

  • Immunoprecipitation - Anti-REG4 antibody [EPR22810-327] (ab255820)
    Immunoprecipitation - Anti-REG4 antibody [EPR22810-327] (ab255820)

    REG4 was immunoprecipitated from 0.35 mg LoVo (human colorectal adenocarcinoma epithelial cell) whole cell lysate 10μg with ab255820 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab255820 1/1000 dilution (0.52 μg/ml). VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

    Lane 1: LoVo (human colorectal adenocarcinoma epithelial cell) whole cell lysate 10μg Lane 2: ab255820 IP in LoVo whole cell lysate
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab255820 in LoVo whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 3 min

    This blot was developed using a higher sensitivity ECL substrate.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REG4 antibody [EPR22810-327] (ab255820)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REG4 antibody [EPR22810-327] (ab255820)

    Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling REG4 with ab255820 at 1/2000 dilution (0.262 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human colon carcinoma (PMID: 25295732) is observed. The section was incubated with ab255820 for 15 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

  • Western blot - Anti-REG4 antibody [EPR22810-327] (ab255820)
    Western blot - Anti-REG4 antibody [EPR22810-327] (ab255820)
    Anti-REG4 antibody [EPR22810-327] (ab255820) at 1/1000 dilution + Recombinant Human REG4 Protein at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 18 kDa
    Observed band size: 14 kDa why is the actual band size different from the predicted?


    Exposure time: 59 seconds


    Blocking/diluting buffer and concentration: 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REG4 antibody [EPR22810-327] (ab255820)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REG4 antibody [EPR22810-327] (ab255820)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling REG4 with ab255820 at 1/2000 dilution (0.262 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human colon (PMID: 25295732) is observed. The section was incubated with ab255820 for 15 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

  • Anti-REG4 antibody [EPR22810-327] (ab255820)
    Anti-REG4 antibody [EPR22810-327] (ab255820)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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