Anti-LRG1/LRG antibody (ab128880)
Key features and details
- Rabbit polyclonal to LRG1/LRG
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-LRG1/LRG antibody
See all LRG1/LRG primary antibodies -
Description
Rabbit polyclonal to LRG1/LRG -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human LRG1/LRG aa 100-200 conjugated to keyhole limpet haemocyanin.
(Peptide available asab129215) -
Positive control
- This antibody gave a positive signal in the following tissue lysates: Human Liver; Human Plasma Total protein; Human Spleen; Human Small Intestine; Human Lung.
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General notes
This product was previously labelled as LRG1
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab128880 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications ChimpanzeeMacaque monkeyGorillaOrangutanApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 38 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 38 kDa).Target
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Tissue specificity
Plasma. -
Sequence similarities
Contains 8 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 116844 Human
- Omim: 611289 Human
- SwissProt: P02750 Human
- Unigene: 655559 Human
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Alternative names
- 1300008B03Rik antibody
- 2310031E04Rik antibody
- A2GL antibody
see all
Images
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Western blot - Anti-LRG1/LRG antibody (ab128880)All lanes : Anti-LRG1/LRG antibody (ab128880) at 1 µg/ml
Lane 1 : Human liver tissue lysate - total protein (ab29889)
Lane 2 : Human Plasma Total Protein Lysate
Lane 3 : Human spleen tissue lysate - total protein (ab29699)
Lane 4 : Human small intestine tissue lysate - total protein (ab29276)
Lane 5 : Lung (Human) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 38 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Additional bands at: 35 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 secondsLRG1/LRG contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
Datasheets and documents
References (0)
ab128880 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-LRG1/LRG antibody (ab128880)All lanes : Anti-LRG1/LRG antibody (ab128880) at 1 µg/ml
Lane 1 : Human liver tissue lysate - total protein (ab29889)
Lane 2 : Human Plasma Total Protein Lysate
Lane 3 : Human spleen tissue lysate - total protein (ab29699)
Lane 4 : Human small intestine tissue lysate - total protein (ab29276)
Lane 5 : Lung (Human) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 38 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Additional bands at: 35 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 secondsLRG1/LRG contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
