Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Fibrinogen alpha chain antibody [EPR2919] - BSA and Azide free (ab247586)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2919] to Fibrinogen alpha chain - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), ICC, IP, IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Fibrinogen alpha chain antibody [EPR2919] - BSA and Azide free
    See all Fibrinogen Alpha Chain primary antibodies

  • Description

    Rabbit monoclonal [EPR2919] to Fibrinogen alpha chain - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    The immunogen is derived from isoform alpha-E, UniProt accession P02671-1, and the antibody is not expected to detect isoform alpha.

  • Tested applications

    Suitable for: Flow Cyt (Intra), ICC, IP, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC: Mouse, rat and human placenta and mouse lung tissue; Flow Cyt (intra): Hepa1-6 cells; WB: Human, mouse and rat plasma lysates. Rat and mouse platelet lysates. Mouse liver tissue lysate; IP: Human plasma; ICC: HepG2 cells.

  • General notes

    ab247586 is the carrier-free version of ab92572.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen alpha chain antibody [EPR2919] - BSA and Azide free (ab247586)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen alpha chain antibody [EPR2919] - BSA and Azide free (ab247586)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placenta tissue sections labeling Fibrinogen alpha chain with purified ab92572 at 1/3000 dilution (0.053 µg/mL). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92572).
  • Western blot - Anti-Fibrinogen alpha chain antibody [EPR2919] - BSA and Azide free (ab247586)
    Western blot - Anti-Fibrinogen alpha chain antibody [EPR2919] - BSA and Azide free (ab247586)
    All lanes : Anti-Fibrinogen alpha chain antibody [EPR2919] (ab92572) at 1/10000 dilution (purified)

    Lane 1 : Human plasma lysate
    Lane 2 : Rat platelet lysate
    Lane 3 : Rat plasma lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 95 kDa
    Observed band size: 95 kDa



    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92572).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen alpha chain antibody [EPR2919] - BSA and Azide free (ab247586)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen alpha chain antibody [EPR2919] - BSA and Azide free (ab247586)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat placenta tissue sections labeling Fibrinogen alpha chain with purified ab92572 at 1/3000 dilution (0.053 µg/mL). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92572).
  • Immunocytochemistry - Anti-Fibrinogen alpha chain antibody [EPR2919] - BSA and Azide free (ab247586)
    Immunocytochemistry - Anti-Fibrinogen alpha chain antibody [EPR2919] - BSA and Azide free (ab247586)

    Immunocytochemistry/ Immunofluorescence analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling Fibrinogen alpha chain with purified ab92572 at 1/50 dilution (3.16 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% TritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92572).

  • Anti-Fibrinogen alpha chain antibody [EPR2919] - BSA and Azide free (ab247586)
    Anti-Fibrinogen alpha chain antibody [EPR2919] - BSA and Azide free (ab247586)

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