Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-LRG1/LRG antibody [EPR12362] (ab178698)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12362] to LRG1/LRG
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-LRG1/LRG antibody [EPR12362]
    See all LRG1/LRG primary antibodies

  • Description

    Rabbit monoclonal [EPR12362] to LRG1/LRG

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    within Human LRG1/LRG aa 250 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: P02750

  • Positive control

    • WB: Human plasma and Human serum lysates;. IHC-P: human liver carcinoma and lung tissue.

  • General notes

     

     

     This product was previously labelled as LRG1

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-LRG1/LRG antibody [EPR12362] (ab178698)
    Western blot - Anti-LRG1/LRG antibody [EPR12362] (ab178698)
    Anti-LRG1/LRG antibody [EPR12362] (ab178698) at 1/10000 dilution (Purified) + Human plasma lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 38 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?



    The band observed is consistent with what has been described in PMID: 28081565

  • Western blot - Anti-LRG1/LRG antibody [EPR12362] (ab178698)
    Western blot - Anti-LRG1/LRG antibody [EPR12362] (ab178698)
    All lanes : Anti-LRG1/LRG antibody [EPR12362] (ab178698) at 1/1000 dilution ((unpurified))

    Lane 1 : Human plasma lysate
    Lane 2 : Human serum lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 38 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRG1/LRG antibody [EPR12362] (ab178698)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRG1/LRG antibody [EPR12362] (ab178698)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver carcinoma tissue sections labeling LRG1/LRG with purified ab178698 at 1/200 dilution (0.57 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRG1/LRG antibody [EPR12362] (ab178698)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRG1/LRG antibody [EPR12362] (ab178698)

    Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling LRG1/LRG with unpurified ab178698 at 1/100 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-LRG1/LRG antibody [EPR12362] (ab178698)
    Anti-LRG1/LRG antibody [EPR12362] (ab178698)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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