Alcian Blue Stain Kit (pH 2.5, Mucin Stain) (ab150662)
Overview
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Product name
Alcian Blue Stain Kit (pH 2.5, Mucin Stain)
See all strongly sulfated and carboxylated mucopolysaccharides & sialomucins kits -
Product overview
Alcian Blue Stain Kit (pH 2.5, Mucin Stain) ab150662 is intended for use in the histological visualization of sulfated and carboxylated acid mucopolysaccharides and sulfated and carboxylated sialomucins (glycoproteins).
This Kit originally used Safranin O Solution but this has now been replaced with Nuclear Fast Red Solution.
Alcian Blue staining protocol summary:
- deparaffinize sections if necessary and hydrate
- incubate in acetic acid for 3 min
- incubate in Alcian Blue pH 2.5 for 30 min
- rinse in water
- stain in nuclear fast red for 5 min
- rinse in water
- dehydrate, clear and mountOther products for staining tissue sections
Find more kits and reagents in the special stains guide, or products for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.
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Notes
Staining Interpretation
Acidic Sulfated Mucosubstances Blue Hyaluronic Acid Blue Sialomucins Blue Nuclei Red Background Pink Control Tissue: Small Intestine, Appendix, Colon
Previously called Alcian Blue pH 2.5 Stain Kit (Mucin Stain)
Properties
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Storage instructions
Store at room temperature. Please refer to protocols. -
Components 100 tests Acetic Acid Solution (3%) 1 x 500ml Alcian Blue pH 2.5 1 x 250ml Nuclear Fast Red Solution 1 x 250ml -
Research areas
Images
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ab150662 - Alcian Blue pH 2.5 Stain Kit (Mucin Stain)
ab150662 Alcian Blue pH 2.5 Stain Kit (Mucin Stain) staining formalin-fixed-paraffin embedded human GI tract.
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ab150662 - Alcian Blue pH 2.5 Stain Kit (Mucin Stain)ab150662 (Alcian Blue pH2.5 stain) staining acidic sulphated mucins (blue) in formalin fixed paraffin embedded normal human colon, counterstained with safranin o (pink).
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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ab150662 - Alcian Blue pH 2.5 Stain Kit (Mucin Stain)Staining of Colon using ab150662 - Alcian Blue pH 2.5 Stain Kit (Mucin Stain).
