Alcian Blue Stain Kit (pH 1.0, Mucin Stain) (ab150661)
Overview
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Product name
Alcian Blue Stain Kit (pH 1.0, Mucin Stain) -
Product overview
The Alcian Blue (pH 1.0) (Mucin Stain) is intended for use in the histological visualization of strongly sulfated mucosubstances.
The component Acetic Acid Solution has been replaced with Hydrochloric Acid Solution in this kit.
Alcian Blue staining protocol summary:
- deparaffinize sections if necessary and hydrate
- prepare Working Rinse solution
- apply Working Rinse solution to tissue for 30 sec
- incubate in Alcian Blue pH 1.0 for 30 min
- rinse in Working Rinse Solution
- blot and air dry slide
- stain in nuclear fast red for 1-2 min
- rinse in water and allow to dry completely
- clear and mountOther products for staining tissue sections
Find more kits and reagents in the special stains guide, or products for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.
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Notes
Staining Interpretation
Strongly Sulfated Mucosubstances Blue Nuclei Red Background Pink Control Tissue: Small Intestine, Appendix, Colon
Previously called Alcian Blue pH 1.0 Stain Kit (Mucin Stain)
Properties
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Storage instructions
Store at room temperature. Please refer to protocols. -
Components 100 tests 1M Hydrochloric Acid Solution 1 x 250ml Alcian Blue pH 1 1 x 250ml Nuclear Fast Red Solution (Enhanced) 1 x 250ml -
Research areas
Images
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ab150661 - Alcian Blue pH 1.0 Stain Kit (Mucin Stain)
ab150661 Alcian Blue pH 1 Stain Kit (Mucin Stain) staining formalin-fixed-paraffin embedded human GI tract.
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ab150661 - Alcian Blue pH 1.0 Stain Kit (Mucin Stain)ab150661 (Alcian Blue pH 1.0) staining strongly sulphated mucins (blue) in formalin fixed paraffin embedded human colon, counterstained with safranin o (pink).
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.
