Anti-SAMM50/SAM50 antibody [EPR8718] (ab133709)
![Anti-SAMM50/SAM50 antibody [EPR8718] (ab133709)](https://www.abcam.com/ps/products/133/ab133709/Images/ab133709-147739-anti-samm50-antibody-epr8718-western-blot.jpg "Anti-SAMM50/SAM50 antibody [EPR8718] (ab133709)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8718] to SAMM50/SAM50
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-SAMM50/SAM50 antibody [EPR8718]
See all SAMM50/SAM50 primary antibodies -
Description
Rabbit monoclonal [EPR8718] to SAMM50/SAM50 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
corresponding to Human SAMM50/SAM50 aa 350-450.
Database link: Q9Y512 -
Positive control
- WB: Human fetal heart lysate, A431 cell lysate, HeLa cell lysate, HepG2 cell lysate IHC-P: Human kidney tissue, Human ovarian carcinoma tissue
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General notes
This product was previously labelled as SAMM50
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 1.30 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR8718 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SAMM50/SAM50 antibody [EPR8718] (ab133709)All lanes : Anti-SAMM50/SAM50 antibody [EPR8718] (ab133709) at 1/1000 dilution
Lane 1 : Human fetal heart lysate
Lane 2 : A431 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 52 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAMM50/SAM50 antibody [EPR8718] (ab133709)](https://www.abcam.com/ps/products/133/ab133709/Images/ab133709-147738-anti-samm50-antibody-epr8718-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAMM50/SAM50 antibody [EPR8718] (ab133709)ab133709, at 1/50 dilution, staining SAMM50/SAM50 in paraffin embedded Human kidney tissue using immunohistochemical analysis.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAMM50/SAM50 antibody [EPR8718] (ab133709)](https://www.abcam.com/ps/products/133/ab133709/Images/ab133709-147737-anti-samm50-antibody-epr8718-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAMM50/SAM50 antibody [EPR8718] (ab133709)ab133709, at 1/50 dilution, staining SAMM50/SAM50 in paraffin embedded Human ovarian carcinoma tissue using immunohistochemical analysis.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![OI-RD Scanning - Anti-SAMM50/SAM50 antibody [EPR8718] (ab133709)](https://www.abcam.com/ps/products/133/ab133709/Images/ab133709-147736-anti-samm50-antibody-epr8718.jpg)
OI-RD Scanning - Anti-SAMM50/SAM50 antibody [EPR8718] (ab133709) -
![Anti-SAMM50/SAM50 antibody [EPR8718] (ab133709)](https://www.abcam.com/ps/products/133/ab133709/Images/ab133709-5-benefits-of-recombinant-antibodies.png)
Anti-SAMM50/SAM50 antibody [EPR8718] (ab133709)
