Anti-MTHFD2 antibody (ab228757)
Key features and details
- Rabbit polyclonal to MTHFD2
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-MTHFD2 antibody
See all MTHFD2 primary antibodies -
Description
Rabbit polyclonal to MTHFD2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow
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Immunogen
Recombinant fragment within Human MTHFD2 (internal sequence). The exact sequence is proprietary.
Database link: P13995 -
Positive control
- WB: A549, HEK-293T, A431, HeLa, HepG2 whole cell lysate. Rat lung and mouse pancreas cell extract. IHC-P: Human colon carcinoma tissue. ICC/IF: HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTHFD2 antibody (ab228757)
Paraffin-embedded human colon carcinoma tissue stained for MTHFD2 protein using ab228757 at 1/250 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-MTHFD2 antibody (ab228757)Methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for MTHFD2 protein (green) using ab228757 at 1/500 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue).
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Western blot - Anti-MTHFD2 antibody (ab228757)Anti-MTHFD2 antibody (ab228757) at 1/1000 dilution + Rat lung whole cell extract at 50 µg
Predicted band size: 38 kDa10% SDS-PAGE
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Western blot - Anti-MTHFD2 antibody (ab228757)All lanes : Anti-MTHFD2 antibody (ab228757) at 1/1000 dilution
Lane 1 : HEK-293T whole cell extracts
Lane 2 : A431 whole cell extracts
Lane 3 : HeLa whole cell extracts
Lane 4 : HepG2 whole cell extracts
Lysates/proteins at 30 µg per lane.
Predicted band size: 38 kDa10% SDS-PAGE
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Western blot - Anti-MTHFD2 antibody (ab228757)Anti-MTHFD2 antibody (ab228757) at 1/1000 dilution + Rat lung whole cell extract at 50 µg
Predicted band size: 38 kDa10% SDS-PAGE
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Western blot - Anti-MTHFD2 antibody (ab228757)Anti-MTHFD2 antibody (ab228757) at 1/1000 dilution + Mouse pancreas whole cell extract at 50 µg
Predicted band size: 38 kDa10% SDS-PAGE
