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Signal Transduction Metabolism Mitochondrial

Anti-SAMM50/SAM50 antibody (ab235308)

Anti-SAMM50/SAM50 antibody (ab235308)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to SAMM50/SAM50
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-SAMM50/SAM50 antibody
    See all SAMM50/SAM50 primary antibodies
  • Description

    Rabbit polyclonal to SAMM50/SAM50
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow
  • Immunogen

    Recombinant fragment corresponding to Human SAMM50/SAM50 aa 1-250.
    Database link: Q9Y512

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: Mouse gonadal lysate; A-375, A431, HEK-293T, HeLa, A549, HepG2, U-251 MG and LO2 whole cell lysates. IHC-P: Human liver cancer and skeletal muscle tissues.
  • General notes

     This product was previously labelled as SAMM50

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers

Images

  • Western blot - Anti-SAMM50/SAM50 antibody (ab235308)
    Western blot - Anti-SAMM50/SAM50 antibody (ab235308)
    All lanes : Anti-SAMM50/SAM50 antibody (ab235308) at 1/1000 dilution

    Lane 1 : Mouse gonadal lysate
    Lane 2 : A-375 (human malignant melanoma cell line) whole cell lysate
    Lane 3 : A431 (human epidermoid carcinoma cell line) whole cell lysate
    Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 5 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 6 : A549 (human lung carcinoma cell line) whole cell lysate
    Lane 7 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 8 : U-251 MG (human brain glioma cell line) whole cell lysate
    Lane 9 : LO2 (human immortal liver cell line) whole cell lysate

    Secondary
    All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution

    Predicted band size: 52 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAMM50/SAM50 antibody (ab235308)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAMM50/SAM50 antibody (ab235308)

    Paraffin-embedded human liver cancer tissue stained for SAMM50/SAM50 using ab235308 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAMM50/SAM50 antibody (ab235308)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAMM50/SAM50 antibody (ab235308)

    Paraffin-embedded human skeletal muscle tissue stained for SAMM50/SAM50 using ab235308 at 1/100 dilution in immunohistochemical analysis.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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    Applications: Flow Cyt, IHC-P, WB

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