Human IGFBP3 Matched Antibody Pair Kit (ab217607)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Human
- Range: 62.5 pg/ml - 4000 pg/ml
Overview
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Product name
Human IGFBP3 Matched Antibody Pair Kit
See all IGFBP3 kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
28.3 pg/ml -
Range
62.5 pg/ml - 4000 pg/ml -
Species reactivity
Reacts with: Human -
Product overview
Human IGFBP3 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse IGFBP3.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab211652. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Human IGFBP3 Capture Antibody 2 x 50µg 1 x 50µg Human IGFBP3 Detector Antibody 2 x 12.5µg 1 x 12.5µg Human IGFBP3 Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. -
Tissue specificity
Expressed by most tissues. Present in plasma. -
Sequence similarities
Contains 1 IGFBP N-terminal domain.
Contains 1 thyroglobulin type-1 domain. -
Developmental stage
IGFBP3 levels are higher during extrauterine life and peak during puberty. -
Domain
The thyroglobulin type-1 domain mediates interaction with HN. -
Post-translational
modificationsPhosphorylation sites are present in the extracelllular medium. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Acid stable subunit of the 140 K IGF complex
- Binding protein 29
- Binding protein 53
see all -
Database links
- Entrez Gene: 3486 Human
- Omim: 146732 Human
- SwissProt: P17936 Human
- Unigene: 450230 Human
- Unigene: 697535 Human
Images
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Human IGFBP3 standard curve.
Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Human IGFBP3 Matched Antibody Pair Kit (ab217607)To learn more about the advantages of recombinant antibodies see here.
