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Human IGFBP3 ELISA Kit (ab211652)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 86.6 pg/ml
  • Range: 122 pg/ml - 7800 pg/ml
  • Sample type: Cell culture supernatant, Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human IGFBP3 ELISA Kit
    See all IGFBP3 kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 8 3.1%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 6.5%

  • Sample type

    Cell culture supernatant, Serum, Plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    86.6 pg/ml

  • Range

    122 pg/ml - 7800 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 100 97% - 103%
    Serum 110 107% - 114%
    Hep Plasma 96 85% - 106%
    EDTA Plasma 104 104% - 104%
    Cit plasma 104 98% - 109%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Human IGFBP3 ELISA Kit (ab211652) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IGFBP3 protein in cell culture supernatant, plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IGFBP3 with 86.6 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Insulin like growth factor binding protein 3 (IGFBP3) belongs to the family of insulin like growth factor (IGF) binding proteins. IGFBP3 is the main IGF binding protein in plasma, and forms a complex with IGF-I or IGF-II and an acid-labile subunit. IGFBP3 is expressed in multiple tissues, where together with IGFs, they play important roles in glucose homeostasis, cell survival, cell growth and differentiation. The concentrations of IGFBP3 are altered in disease states like type I diabetes, where urinary levels are increased during microalbuminuria, and in prostate cancer, where IGFBP3 protein levels decrease.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Images

  • Other - Human IGFBP3 ELISA Kit (ab211652)
    Other - Human IGFBP3 ELISA Kit (ab211652)

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Human IGFBP3 standard curve comparison data.
    Human IGFBP3 standard curve comparison data.

    Standard curve comparison between human IGFBP3 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows comparable sensitivity.

  • Example of human IGFBP3 standard curve in Sample Diluent NS.
    Example of human IGFBP3 standard curve in Sample Diluent NS.

    Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated concentrations of native IGFBP3 in human serum and plasma samples.
    Interpolated concentrations of native IGFBP3 in human serum and plasma samples.

    The concentrations of IGFBP3 were measured in duplicates, interpolated from the IGFBP3 standard curves and corrected for sample dilution. Undiluted samples are as follows:  serum 1:300, plasma (citrate) 1:300, plasma (heparin) 1:333, and plasma (EDTA) 1:500.  The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).  The mean IGFBP3 concentration was determined to be 2,161 ng/mL in serum, 2,175 ng/mL in plasma (citrate), 822 ng/mL in plasma (heparin), and 1,944 ng/mL in plasma (EDTA).

  • Interpolated concentrations of native IGFBP3 in SH-SY5Y supernatant samples.
    Interpolated concentrations of native IGFBP3 in SH-SY5Y supernatant samples.

    The concentrations of IGFBP3 were measured in duplicates, interpolated from the IGFBP3 standard curves and corrected for sample dilution. Undiluted samples are as follows: SH-SY5Y supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGFBP3 concentration was determined to be 4.28 ng/ml in SH-SY5Y supernatant.

  • Serum from ten individual healthy human male donors was measured in duplicate.
    Serum from ten individual healthy human male donors was measured in duplicate.

    Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).  The mean IGFBP3 concentration was determined to be 1442 ng/mL with a range of 104 – 3,095 ng/mL.

  • Sandwich ELISA - Human IGFBP3 ELISA Kit (ab211652)
    Sandwich ELISA - Human IGFBP3 ELISA Kit (ab211652)
    To learn more about the advantages of recombinant antibodies see here.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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