Anti-LDB1 antibody (ab96799)
Key features and details
- Rabbit polyclonal to LDB1
- Suitable for: WB, IHC-P, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-LDB1 antibody
See all LDB1 primary antibodies -
Description
Rabbit polyclonal to LDB1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P RatHumanIP HumanWB MouseHuman
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Immunogen
Recombinant fragment corresponding to a region within amino acids 50 and 345 of Human LDB1 (NP_003884)
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Positive control
- WB: HEK293T, A431, HeLa, HepG2 and Raji cell lysates; Mouse brain tissue lysate. IHC-P: U87 xenograft; Mouse muscle and brain tissues. IHC-Fr: Mouse brain tissue. ICC/IF: HeLa cells. IP: HEK293T cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-LDB1 antibody (ab96799)
HeLa cells stained for LDB1 (green) using ab96799 at 1/500 dilution in ICC/IF.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LDB1 antibody (ab96799)Immunohistochemical analysis of paraffin-embedded U87 xenograft using ab96799 at a dilution of 1/100. -
Western blot - Anti-LDB1 antibody (ab96799)Anti-LDB1 antibody (ab96799) at 1/1000 dilution + Mouse brain lysate at 50 µg
Predicted band size: 47 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-LDB1 antibody (ab96799)Immunofluorescence analysis of paraformaldheyde-fixed HeLa cells using ab96799 at a dilution of 1/200. -
Western blot - Anti-LDB1 antibody (ab96799)All lanes : Anti-LDB1 antibody (ab96799) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 47 kDa
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Western blot - Anti-LDB1 antibody (ab96799)Anti-LDB1 antibody (ab96799) at 1/1000 dilution + Raji whole cell lysate at 30 µg
Predicted band size: 47 kDa
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Immunoprecipitation - Anti-LDB1 antibody (ab96799)LDB1 was immunoprecipitated from 1000 µg 293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate with ab96799. Western blot was performed from the immunoprecipitate using 2.5 μg of ab96799 at 1/1000 dilution.
Lane A: 20 μg 293T whole cell lysate/extract.
Lane B: Control with 2.5 μg of preimmune rabbit IgG.
Lane C: ab96799 IP in 293T (12% SDS-PAGE). -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LDB1 antibody (ab96799)Paraffin-embedded mouse muscle tissue stained for LDB1 using ab96799 at 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LDB1 antibody (ab96799)Paraffin-embedded rat middle brain tissue stained for LDB1 using ab96799 at a/500 dilution in immunohistochemical analysis.
