Anti-CRMP3 antibody (ab36217)
Key features and details
- Rabbit polyclonal to CRMP3
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-CRMP3 antibody
See all CRMP3 primary antibodies -
Description
Rabbit polyclonal to CRMP3 -
Host species
Rabbit -
Specificity
No cross reactivity was detected with CRMP1, CRMP2, CRMP4 or CRMP5. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
-
Immunogen
A 15 residue synthetic peptide derived from C-terminal domain of human CRMP3.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: Whole serum -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-CRMP3 antibody (ab36217)All lanes : Anti-CRMP3 antibody (ab36217)
Lane 1 : E.coli transformed with cDNA of human CRMP1
Lane 2 : E.coli transformed with cDNA of human CRMP2
Lane 3 : E.coli transformed with cDNA of human CRMP3
Lane 4 : E.coli transformed with cDNA of human CRMP4
Lane 5 : E.coli transformed with cDNA of human CRMP5
Predicted band size: 65 kDa
Observed band size: 65 kDa
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRMP3 antibody (ab36217)IHC image of ab36217 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab36217, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
