Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-DYRK1A antibody [EPR24132-61] - BSA and Azide free (ab280359)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24132-61] to DYRK1A - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-DYRK1A antibody [EPR24132-61] - BSA and Azide free
    See all DYRK1A primary antibodies

  • Description

    Rabbit monoclonal [EPR24132-61] to DYRK1A - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-Fr
    Human
    WB
    Mouse
    Human
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, HeLa transfected with scrambled siRNA control, DYRK1A knockdown HeLa, 293T, NIH/3T3, PC-12, Mouse E17 brain, P1 brain and P240 brain lysates.

  • General notes

    ab280359 is the carrier-free version of ab259869. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab280359 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    Constituent: 100% PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR24132-61

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-DYRK1A antibody [EPR24132-61] - BSA and Azide free (ab280359)
    Western blot - Anti-DYRK1A antibody [EPR24132-61] - BSA and Azide free (ab280359)
    All lanes : Anti-DYRK1A antibody [EPR24132-61] (ab259869) at 1/1000 dilution

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate
    Lane 2 : DYRK1A knockdown HeLa whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/20000 dilution

    Predicted band size: 86 kDa
    Observed band size: 90 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab259869, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    This antibody may react with an unidentifiable protein around 30 kDa.

    Exposure time: 62 seconds

  • Western blot - Anti-DYRK1A antibody [EPR24132-61] - BSA and Azide free (ab280359)
    Western blot - Anti-DYRK1A antibody [EPR24132-61] - BSA and Azide free (ab280359)
    All lanes : Anti-DYRK1A antibody [EPR24132-61] (ab259869) at 1/1000 dilution

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : 293T (human embryonic kidney epithelial cell) whole cell lysate
    Lane 3 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
    Lane 4 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 86 kDa
    Observed band size: 90 kDa why is the actual band size different from the predicted?



    This data was developed using ab259869, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Lysates were made freshly and used in WB test immediately to minimize protein degradation.

    Exposure time: 70 seconds

  • Western blot - Anti-DYRK1A antibody [EPR24132-61] - BSA and Azide free (ab280359)
    Western blot - Anti-DYRK1A antibody [EPR24132-61] - BSA and Azide free (ab280359)
    All lanes : Anti-DYRK1A antibody [EPR24132-61] (ab259869) at 1/1000 dilution

    Lane 1 : Mouse E17 brain tissue lysate
    Lane 2 : Mouse P1 brain tissue lysate
    Lane 3 : Mouse P240 brain tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 86 kDa
    Observed band size: 90 kDa why is the actual band size different from the predicted?



    This data was developed using ab259869, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The expression profile observed is consistent with what has been described in the literature (PMID:28167836).

    Exposure time: 15 seconds

  • Anti-DYRK1A antibody [EPR24132-61] - BSA and Azide free (ab280359)
    Anti-DYRK1A antibody [EPR24132-61] - BSA and Azide free (ab280359)

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