Anti-DYRK1A antibody - C-terminal (ab180910)
Key features and details
- Rabbit polyclonal to DYRK1A - C-terminal
- Suitable for: ICC, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-DYRK1A antibody - C-terminal
See all DYRK1A primary antibodies -
Description
Rabbit polyclonal to DYRK1A - C-terminal -
Host species
Rabbit -
Specificity
ab180910 will not cross-react with other DYRK family members. -
Tested applications
Suitable for: ICC, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide corresponding to Human DYRK1A (C terminal). (17 amino acid peptide; NP_001387).
Database link: Q13627 -
Positive control
- HeLa cell lysate; HeLa cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot analysis of DYRK1A expression in K562 cell lysate with ab180910 at (A) 1 and (B) 2µg/ml.
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Western Blot of HeLa cell lysate labeling DYRK1A with Anti-DYRK1A antibody - C-terminal (ab180910) at (1) 1 and (2) 2µg/ml.
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All lanes : Anti-DYRK1A antibody - C-terminal (ab180910) at 1 µg/ml
Lane 1 : K562 cell lysate
Lane 2 : Daudi cell lysate
Lane 3 : EL4 cell lysate (mouse cell line)
Predicted band size: 86 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?15ug protein per lane
Blocking buffer: 5% non-fat dry milk in TBST
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Immunocytochemical analysis of HeLa cells labeling DYRK1A with ab180910 at 10 µg/ml.
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Lane 1 : Anti-DYRK1A antibody - C-terminal (ab180910) at 1 µg/ml
Lane 2 : Anti-DYRK1A antibody - C-terminal (ab180910) at 2 µg/ml
All lanes : HeLa cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 86 kDa