Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Wednesday March 10, 2021

Anti-PTF1A antibody [EPR19011] (ab182398)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR19011] to PTF1A
Suitable for: IP, WB
Reacts with: Mouse, Rat, Human

Product name

Anti-PTF1A antibody [EPR19011]
See all PTF1A primary antibodies
Description

Rabbit monoclonal [EPR19011] to PTF1A
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Mouse

WB
Mouse

Rat

Human

See all applications and species data
Immunogen

Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human fetal heart and fetal kidney lysates; BxPC-3, C6, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates; Rat heart and pancreas lysates; Mouse pancreas lysate. IP: NIH/3T3 whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IP	Mouse
WB	Mouse Rat Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR19011
Isotype

IgG
Research areas

Western blot - Anti-PTF1A antibody [EPR19011] (ab182398)

All lanes : Anti-PTF1A antibody [EPR19011] (ab182398) at 1/2000 dilution

Lane 1 : Human fetal heart lysate
Lane 2 : Human fetal kidney lysate
Lane 3 : BxPC-3 (Human pancreas adenocarcinoma cell line) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/10000 dilution

Predicted band size: 35 kDa
Observed band size: 42 kDa
why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID: 18347078).
Western blot - Anti-PTF1A antibody [EPR19011] (ab182398)

All lanes : Anti-PTF1A antibody [EPR19011] (ab182398) at 1/2000 dilution

Lane 1 : Rat heart lysate at 20 µg
Lane 2 : Rat pancreas lysate at 20 µg
Lane 3 : C6 (Rat glial tumor cell line) whole cell lysate at 10 µg
Lane 4 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg
Lane 5 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 10 µg
Lane 6 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 35 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure times: Lane 1: 3 minutes; Lane 2: 30 seconds; Lane 3, 4, 5 and 6: 10 seconds.
Western blot - Anti-PTF1A antibody [EPR19011] (ab182398)

Anti-PTF1A antibody [EPR19011] (ab182398) at 1/5000 dilution + Mouse pancreas lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 35 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunoprecipitation - Anti-PTF1A antibody [EPR19011] (ab182398)

PTF1A was immunoprecipitated from 1 mg of NIH/3T3 (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab182398 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab182398 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1: NIH/3T3 whole cell lysate 10 μg (Input).

Lane 2: ab182398 IP in NIH/3T3 whole cell lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab182398 in NIH/3T3 whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 10 seconds.
Anti-PTF1A antibody [EPR19011] (ab182398)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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