Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PTF1A antibody [EPR19011] (ab182398)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19011] to PTF1A
  • Suitable for: IP, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PTF1A antibody [EPR19011]
    See all PTF1A primary antibodies

  • Description

    Rabbit monoclonal [EPR19011] to PTF1A

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Mouse
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human fetal heart and fetal kidney lysates; BxPC-3, C6, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates; Rat heart and pancreas lysates; Mouse pancreas lysate. IP: NIH/3T3 whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-PTF1A antibody [EPR19011] (ab182398)
    Western blot - Anti-PTF1A antibody [EPR19011] (ab182398)
    All lanes : Anti-PTF1A antibody [EPR19011] (ab182398) at 1/2000 dilution

    Lane 1 : Human fetal heart lysate
    Lane 2 : Human fetal kidney lysate
    Lane 3 : BxPC-3 (Human pancreas adenocarcinoma cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/10000 dilution

    Predicted band size: 35 kDa
    Observed band size: 42 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The molecular weight  observed is consistent with what has been described in the literature (PMID: 18347078).

  • Western blot - Anti-PTF1A antibody [EPR19011] (ab182398)
    Western blot - Anti-PTF1A antibody [EPR19011] (ab182398)
    All lanes : Anti-PTF1A antibody [EPR19011] (ab182398) at 1/2000 dilution

    Lane 1 : Rat heart lysate at 20 µg
    Lane 2 : Rat pancreas lysate at 20 µg
    Lane 3 : C6 (Rat glial tumor cell line) whole cell lysate at 10 µg
    Lane 4 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg
    Lane 5 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 10 µg
    Lane 6 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 35 kDa
    Observed band size: 42 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1: 3 minutes; Lane 2: 30 seconds; Lane 3, 4, 5 and 6: 10 seconds.

  • Western blot - Anti-PTF1A antibody [EPR19011] (ab182398)
    Western blot - Anti-PTF1A antibody [EPR19011] (ab182398)
    Anti-PTF1A antibody [EPR19011] (ab182398) at 1/5000 dilution + Mouse pancreas lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 35 kDa
    Observed band size: 42 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-PTF1A antibody [EPR19011] (ab182398)
    Immunoprecipitation - Anti-PTF1A antibody [EPR19011] (ab182398)

    PTF1A was immunoprecipitated from 1 mg of NIH/3T3 (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab182398 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab182398 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: NIH/3T3 whole cell lysate 10 μg (Input).

    Lane 2: ab182398 IP in NIH/3T3 whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab182398 in NIH/3T3 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

  • Anti-PTF1A antibody [EPR19011] (ab182398)
    Anti-PTF1A antibody [EPR19011] (ab182398)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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