Anti-Hes1 antibody [2D2] (ab119776)
![Anti-Hes1 antibody [2D2] (ab119776)](https://www.abcam.com/ps/products/119/ab119776/Images/ab119776-133030-anti-hes1-antibody-2d2-western-blot.jpg "Anti-Hes1 antibody [2D2] (ab119776)")
Key features and details
- Mouse monoclonal [2D2] to Hes1
- Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
- Knockout validated
- Reacts with: Human, African green monkey
- Isotype: IgG1
Overview
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Product name
Anti-Hes1 antibody [2D2]
See all Hes1 primary antibodies -
Description
Mouse monoclonal [2D2] to Hes1 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanAfrican green monkeyIHC-P HumanWB Human
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Immunogen
Recombinant full length Human Hes1 produced in HEK293T cells (NP_005515).
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Positive control
- WB: HEK293T cell lysate transfected with pCMV6-ENTRY Hes1 cDNA. ICC/IF: MCF7 cell lysates; COS7 cells transiently transfected by pCMV6-ENTRY Hes1 cDNA. Flow Cyt: Jurkat cells. IHC-P: Human thyroid, thyroid carcinoma and bladder carcinoma tissues.
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General notes
Dilute in PBS (pH7.3) before use.
This product was changed from ascites to tissue culture supernatant on the 4th of June 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol -
Concentration information loading... -
Purity
Tissue culture supernatant -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
2D2 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-Hes1 antibody [2D2] (ab119776)All lanes : Anti-Hes1 antibody [2D2] (ab119776) at 1/2000 dilution
Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY Hes1 cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 30 kDaHEK293T cell lysates were generated from transient transfection of the cDNA clone (RC211709)
This image was generated using the ascites version of the product.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hes1 antibody [2D2] (ab119776)](https://www.abcam.com/ps/products/119/ab119776/Images/ab119776-133027-anti-hes1-antibody-2d2-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hes1 antibody [2D2] (ab119776)ab119776, at 1/150 dilution, staining Hes1 in paraffin-embedded Human bladder carcinoma tissue by Immunohistochemistry.
This image was generated using the ascites version of the product.
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![Immunocytochemistry/ Immunofluorescence - Anti-Hes1 antibody [2D2] (ab119776)](https://www.abcam.com/ps/products/119/ab119776/Images/ab119776-7-anti-hes1-antibody-2d2-immunocytochemistry-immunofluorescence-mcf7-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Hes1 antibody [2D2] (ab119776)ab119776 staining HES1 in wild-type MCF7 cells (top panel) and HES1 knockout MCF7 cells (bottom panel). The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab119776 at 1/500 dilution and ab7291 (Mouse monoclonal to alpha Tubulin) at 1/1000 dilution overnight at 4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to mouse IgG (Alexa Fluor® 488) (ab150117) at 2 µg/ml (shown in green) and a goat secondary antibody to rabbit IgG (Alexa Fluor® 594) (ab150080) at 2 µg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hes1 antibody [2D2] (ab119776)](https://www.abcam.com/ps/products/119/ab119776/Images/ab119776-133026-anti-hes1-antibody-2d2-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hes1 antibody [2D2] (ab119776)ab119776, at 1/150 dilution, staining Hes1 in paraffin-embedded Human thyroid carcinoma tissue by Immunohistochemistry.
This image was generated using the ascites version of the product.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hes1 antibody [2D2] (ab119776)](https://www.abcam.com/ps/products/119/ab119776/Images/ab119776-133025-anti-hes1-antibody-2d2-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hes1 antibody [2D2] (ab119776)ab119776, at 1/150 dilution, staining Hes1 in paraffin-embedded Human thyroid tissue by Immunohistochemistry.
This image was generated using the ascites version of the product.
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![Immunocytochemistry/ Immunofluorescence - Anti-Hes1 antibody [2D2] (ab119776)](https://www.abcam.com/ps/products/119/ab119776/Images/ab119776-133029-anti-hes1-antibody-2d2-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Hes1 antibody [2D2] (ab119776)ab119776, at 1/100 dilution, staining Hes1 in COS7 cells transiently transfected with pCMV6-ENTRY Hes1 cDNA by Immunofluorescence.
This image was generated using the ascites version of the product.
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![Flow Cytometry - Anti-Hes1 antibody [2D2] (ab119776)](https://www.abcam.com/ps/products/119/ab119776/Images/ab119776-133028-anti-hes1-antibody-2d2-flow-cytometry.jpg)
Flow Cytometry - Anti-Hes1 antibody [2D2] (ab119776)ab119776, at 1/100 dilution, staining Hes1 (red) in Jurkat cells, compared to a nonspecific negative control antibody,(blue) by Flow cytometry.
This image was generated using the ascites version of the product.
