All lanes : Anti-ID2 antibody [OTI10C3] (ab90055) at 1/500 dilution

Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells were transfected with the pCMV6-ENTRY control
Lane 2 : HEK-293T cells were transfected with the pCMV6-ENTRY ID2 cDNA

Lysates/proteins at 5 µg per lane.

Predicted band size: 15 kDa

Paraffin-embedded human bladder carcinoma tissue stained for ID2 with ab90055 at a 1/50 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.

HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for ID2 using ab90055 at a 1/50 dilution in ICC/IF.

Paraffin-embedded human liver tissue stained for ID2 with ab90055 at a 1/50 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.

Paraffin-embedded human liver carcinoma tissue stained for ID2 with ab90055 at a 1/50 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.

Overlay histogram showing SH-SY5Y cells stained with ab90055 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab90055, 1µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.