Anti-ID2 antibody [OTI9A8] (ab236504)
![Anti-ID2 antibody [OTI9A8] (ab236504)](https://www.abcam.com/ps/products/236/ab236504/Images/ab236504-312864-anti-id2-antibody-oti9a8-western-blot.jpg "Anti-ID2 antibody [OTI9A8] (ab236504)")
Key features and details
- Mouse monoclonal [OTI9A8] to ID2
- Suitable for: IHC-P, WB
- Reacts with: Human, Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-ID2 antibody [OTI9A8]
See all ID2 primary antibodies -
Description
Mouse monoclonal [OTI9A8] to ID2 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanRecombinant fragmentWB Human
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Immunogen
Recombinant full length protein corresponding to Human ID2 aa 1 to the C-terminus. (NP_002157) produced in E.coli.
Database link: Q02363 -
Positive control
- WB: HEK-293T transfected with pCMV6-ENTRY ID2 whole cell lysate. IHC-P: Human liver carcinoma tissue. ICC/IF: COS-7 cells transiently transfected with pCMV6-ENTRY ID2.
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General notes
Clone OTI9A8 (formerly 9A8).
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Purified from cell culture supernatant. -
Clonality
Monoclonal -
Clone number
OTI9A8 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-ID2 antibody [OTI9A8] (ab236504)All lanes : Anti-ID2 antibody [OTI9A8] (ab236504) at 1/500 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with pCMV6-ENTRY control, cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with pCMV6-ENTRY ID2, cell lysate
Lysates/proteins at 5 µg per lane.
Predicted band size: 14 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ID2 antibody [OTI9A8] (ab236504)](https://www.abcam.com/ps/products/236/ab236504/Images/ab236504-312865-anti-id2-antibody-oti9a8-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ID2 antibody [OTI9A8] (ab236504)Paraffin-embedded human liver carcinoma tissue stained for ID2 using ab236504 at 1/50 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ID2 antibody [OTI9A8] (ab236504)](https://www.abcam.com/ps/products/236/ab236504/Images/ab236504-312866-anti-id2-antibody-oti9a8-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ID2 antibody [OTI9A8] (ab236504)COS-7 (African green monkey kidney fibroblast-like cell line) cells transiently transfected with pCMV6-ENTRY ID2 stained for ID2 (green) using ab236504 at 1/100 dilution in ICC/IF.
