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Epigenetics and Nuclear Signaling Transcription Domain Families HLH / Leucine Zipper HLH

Anti-ID2 antibody [4E12G5] (ab166708)

Anti-ID2 antibody [4E12G5] (ab166708)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [4E12G5] to ID2
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-ID2 antibody [4E12G5]
    See all ID2 primary antibodies
  • Description

    Mouse monoclonal [4E12G5] to ID2
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Purified recombinant full length protein, corresponding to amino acids 1-134 of Human ID2, expressed in E. coli (UniProt: Q02363).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Human recombinant ID2 protein; SK N SH cells; Human breast cancer and rectum cancer tissues.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    4E12G5
  • Isotype

    IgG1
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • HLH / Leucine Zipper
    • HLH
    • Stem Cells
    • Signaling Pathways
    • TGF beta
    • Nuclear

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ID2 antibody [4E12G5] (ab166708)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ID2 antibody [4E12G5] (ab166708)
    Immunohistochemical analysis of paraffin embedded, DAB-stained Human breast cancer tissue labeling ID2 using ab166708 at a 1/200 dilution.
  • Western blot - Anti-ID2 antibody [4E12G5] (ab166708)
    Western blot - Anti-ID2 antibody [4E12G5] (ab166708)
    Anti-ID2 antibody [4E12G5] (ab166708) at 1/500 dilution + recombinant Human ID2 protein at 1 µg

    Predicted band size: 17.3 kDa

  • Flow Cytometry - Anti-ID2 antibody [4E12G5] (ab166708)
    Flow Cytometry - Anti-ID2 antibody [4E12G5] (ab166708)
    Flow cytometric analysis of ID2-labeled SK N SH cells (green) and negative control cells (red), using ab166708 at a 1/200 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ID2 antibody [4E12G5] (ab166708)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ID2 antibody [4E12G5] (ab166708)
    Immunohistochemical analysis of paraffin embedded, DAB-stained Human rectum cancer tissue labeling ID2 using ab166708 at a 1/200 dilution.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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