Human BMP-4 ELISA Kit (ab231930)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 0.28 pg/ml
- Range: 12 pg/ml - 750 pg/ml
- Sample type: Cell culture extracts, Cell culture supernatant, Cit plasma, EDTA Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human BMP-4 ELISA Kit
See all BMP4 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% HT-29 S/N 5 6.3% Inter-assay Sample n Mean SD CV% HT-29 S/N 3 4.9% -
Sample type
Cell culture supernatant, Serum, Cell culture extracts, Tissue Extracts, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.28 pg/ml -
Range
12 pg/ml - 750 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 89 86% - 91% Cell culture extracts 104 97% - 108% Tissue Extracts 92 87% - 99% Cell culture media 118 110% - 125% EDTA Plasma 85 80% - 90% Cit plasma 87 82% - 93% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human
Predicted to work with: Cow, Rhesus monkey
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Product overview
Human BMP-4 ELISA Kit (ab231930) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of BMP-4 protein in cell culture extracts, cell culture supernatant, cit plasma, edta plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human BMP-4 with NULL NULL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human BMP-4 Capture Antibody 1 x 600µl 10X Human BMP-4 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BI 1 x 6ml Human BMP-4 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Induces cartilage and bone formation. Also act in mesoderm induction, tooth development, limb formation and fracture repair. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction. -
Tissue specificity
Expressed in the lung and lower levels seen in the kidney. Present also in normal and neoplastic prostate tissues, and prostate cancer cell lines. -
Involvement in disease
Defects in BMP4 are the cause of microphthalmia syndromic type 6 (MCOPS6) [MIM:607932]; also known as microphthalmia and pituitary anomalies or microphthalmia with brain and digit developmental anomalies. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS6 is characterized by microphthalmia/anophthalmia associated with facial, genital, skeletal, neurologic and endocrine anomalies.
Defects in BMP4 are the cause of non-syndromic orofacial cleft type 11 (OFC11) [MIM:600625]. Non-syndromic orofacial cleft is a common birth defect consisting of cleft lips with or without cleft palate. Cleft lips are associated with cleft palate in two-third of cases. A cleft lip can occur on one or both sides and range in severity from a simple notch in the upper lip to a complete opening in the lip extending into the floor of the nostril and involving the upper gum. OFC11 is an unusual anomaly consisting of a paramedian scar of the upper lip with an appearance suggesting that a typical cleft lip was corrected in utero. -
Sequence similarities
Belongs to the TGF-beta family. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Alternative names
- zgc:100779
- BMP 2B
- BMP 4
see all -
Database links
- Entrez Gene: 652 Human
- Omim: 112262 Human
- SwissProt: P12644 Human
- Unigene: 68879 Human
Images
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Other - Human BMP-4 ELISA Kit (ab231930)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Human BMP-4 Competitor standard curve comparisonStandard curve comparison between Human BMP-4 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
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Example of human BMP-4 standard curve in Sample Diluent NS.The BMP-4 standard curve was prepared as described in Section 10. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native BMP-4 in human cell culture supernatant samples.The concentrations of BMP-4 were measured in duplicates, interpolated from the BMP-4 standard curves and corrected for sample dilution. Undiluted samples are as follows: SW480 cell culture supernatant 100%, HT-29 cell culture supernatant 50%, HepG2 cell culture supernatant 100%, and naïve culture media (100% RPMI + 10% FBS) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean BMP-4 concentration was determined to be 535.2 pg/mL in neat SW480 cell culture supernatant, 1035 pg/mL in neat HT-29 cell culture supernatant, 103.3 pg/mL in HepG2 cell culture supernatant, and 24.02 pg/mL in naïve culture media containing 10% FBS.
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Interpolated concentrations of native BMP-4 in human cell and tissue extract.Interpolated concentrations of native BMP-4 in human HT-29 cell extract based on a 150 μg/mL extract load, HepG2 cell extract based on a 300 μg/mL extract load, and colon tissue extract based on a 1,000 μg/mL extract load. The concentrations of BMP-4 were measured in duplicate and interpolated from the BMP-4 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean BMP-4 concentration was determined to be 456.7 pg/mL in HT-29 cell extract, 88.13 pg/mL in HepG2 cell extract, and 22.20 pg/mL in colon tissue extract.
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Interpolated concentrations of native BMP-4 in human serum and plasma samples.The concentrations of BMP-4 were measured in duplicates, interpolated from the BMP-4 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (citrate) 25%, and plasma (EDTA) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean BMP-4 concentration was determined to be 193.9 pg/mL in neat serum, 162.0 pg/mL in neat plasma (citrate), and 91.1 pg/mL in neat plasma (EDTA).
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Serum from ten individual healthy human female donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean BMP-4 concentration in neat serum was determined to be 163.5 pg/mL with a range of 69.95 – 505.8 pg/mL.
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Example of human BMP-4 standard curve in 1X Cell Extraction Buffer PTR.The BMP-4 standard curve was prepared as described in Section 10. Background-subtracted data values (mean +/- SD) are graphed.
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Sandwich ELISA - Human BMP-4 ELISA Kit (ab231930)To learn more about the advantages of recombinant antibodies see here.
